ArticleThe Journal of cell biology1989
The use of retinoic acid to probe the relation between hyperproliferation-associated keratins and cell proliferation in normal and malignant epidermal cells.
Article in The Journal of cell biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 153 citations in OpenAlex.
- Article
- Article
- RARα and RARγ reciprocally control K5Organogenesis · 2017Article
- The genetics of human skin disease.Cold Spring Harbor perspectives in medicine · 2014Review
- Downregulation of STRA6 expression in epidermal keratinocytes leads to hyperproliferation-associated differentiation in both in vitro and in vivo skin models.The Journal of investigative dermatology · 2014Article
- Keratin 16 regulates innate immunity in response to epidermal barrier breach.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- 2,6-Dithiopurine, a nucleophilic scavenger, protects against mutagenesis in mouse skin treated in vivo with 2-(chloroethyl) ethyl sulfide, a mustard gas analog.Toxicology and applied pharmacology · 2012Article
- Differential keratin expression during epiboly in a wound model of bioengineered skin and in human chronic wounds.The international journal of lower extremity wounds · 2011Article
- Actomyosin-mediated cellular tension drives increased tissue stiffness and β-catenin activation to induce epidermal hyperplasia and tumor growth.Cancer cell · 2011Article
- Epidermolysis bullosa simplex: a paradigm for disorders of tissue fragility.The Journal of clinical investigation · 2009Review
- HPV8 early genes modulate differentiation and cell cycle of primary human adult keratinocytes.Experimental dermatology · 2007Article
- The targeted overexpression of a Claudin mutant in the epidermis of transgenic mice elicits striking epidermal and hair follicle abnormalities.Molecular biotechnology · 2007Article
- E6/E7 expression of human papillomavirus type 20 (HPV-20) and HPV-27 influences proliferation and differentiation of the skin in UV-irradiated SKH-hr1 transgenic mice.Journal of virology · 2006Article
- Keratin 6 is not essential for mammary gland development.Breast cancer research : BCR · 2006Article
- Expression of keratin K2e in cutaneous and oral lesions: association with keratinocyte activation, proliferation, and keratinization.The American journal of pathology · 2003Article
- Forced expression of keratin 16 alters the adhesion, differentiation, and migration of mouse skin keratinocytes.Molecular biology of the cell · 2000Article
- Structural cues from the tissue microenvironment are essential determinants of the human mammary epithelial cell phenotype.Journal of mammary gland biology and neoplasia · 1998Review
- The basal keratin network of stratified squamous epithelia: defining K15 function in the absence of K14.The Journal of cell biology · 1995Article
- The relationship of cytoplasmic intermediate filaments and membrane antigens with hormone receptors, nuclear staining density, and mode of stromal invasion in human breast cancer.Breast cancer research and treatment · 1995Article
- Increased expression of keratin 16 causes anomalies in cytoarchitecture and keratinization in transgenic mouse skin.The Journal of cell biology · 1994Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
When cells from normal human epidermis and from the human squamous cell carcinoma line SCC-13 were seeded on floating rafts of collagen and fibroblasts, they stratified and underwent terminal differentiation. Although the program of differentiation in SCC-13 cells was morphologically abnormal, the cultures resembled normal epidermal raft cultures by expressing the terminal differentiation-specific keratins, K1/K10, and by restricting their proliferative capacity to the basal-like cells of the population. In addition, the differentiating cells of both normal and SCC-13 raft cultures expressed keratins K6 and K16, which are not normally expressed in epidermis, but are synthesized suprabasally during wound-healing and in various epidermal diseases associated with hyperproliferation. While the behavior of normal and SCC-13 rafts was quite similar when they were cultured over normal medium, significant biochemical differences began to emerge when the cultures were exposed to retinoic acid. Most notably, while the SCC-13 cultures still stratified extensively, they showed a marked inhibition of both abnormal (K6/K16) and normal (K1/K10) differentiation-associated keratins, concomitantly with an overall disappearance of differentiated phenotype. Surprisingly, the reduction in K6/K16 in retinoid-treated SCC-13 cultures was not accompanied by a decrease in cell proliferation. Using immunohistochemistry combined with [3H]thymidine labeling, we demonstrate that while the expression of K6 and K16 are often associated with hyperproliferation, these keratins are only produced in the nondividing, differentiating populations of proliferating cultures. Moreover, since their expression can be suppressed without a corresponding decrease in proliferation, the expression of these keratins cannot be essential to the nature of the hyperproliferative epidermal cell.
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