Evidence map›Paper›PMID 24729934›Full record

ArticleAging and disease2014

Resveratrol Suppresses Expression of VEGF by Human Retinal Pigment Epithelial Cells: Potential Nutraceutical for Age-related Macular Degeneration.

Chandrasekharam N Nagineni, Raghavan Raju, Krishnasai K Nagineni, Vijay K Kommineni, Aswini Cherukuri, R Krishnan Kutty, John J Hooks, Barbara Detrick

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Aging and disease, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.

0numbers the graph read from it
0cells of the map it votes in
48citing papers in PubMed
9.1field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

48 citing papers in PubMed, 73 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Chandrasekharam N NagineniLaboratory of Immunology, National Eye Institute, NIH, Bethesda, Maryland, USA.
Raghavan RajuDepartments of Medical Laboratory, Imaging and Radiological Sciences, and Biochemistry and Molecular Biology, Georgia Regents University, Augusta, Georgia, USA.
Krishnasai K NagineniSchool of Public Policy, University of Maryland, College Park, Maryland, USA.
Vijay K KommineniLaboratory of Immunology, National Eye Institute, NIH, Bethesda, Maryland, USA.
Aswini CherukuriLaboratory of Retinal Cell and Molecular Biology, National Eye institute, NIH, Bethesda, Maryland.
R Krishnan KuttyLaboratory of Retinal Cell and Molecular Biology, National Eye institute, NIH, Bethesda, Maryland.
John J HooksLaboratory of Immunology, National Eye Institute, NIH, Bethesda, Maryland, USA.
Barbara DetrickDepartment of Pathology, Johns Hopkins University, School of Medicine, Baltimore, Maryland, USA.
National Eye Institute · USJohns Hopkins University · USUniversity of Maryland, College Park · US

Funding

Signaling in the retina and retinal pigment epitheliumZIAEY000444 · NEI · NATIONAL EYE INSTITUTE · PI REDMOND, T. MICHAEL · 2009 to 2025
$23.4M
Resveratrol as an adjunct to resuscitation fluid following hemorrhage injuryR01GM101927 · NIGMS · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI RAJU, RAGHAVAN PILLAI · 2012 to 2016
$1.1M
NIGMS NIH HHS R01 GM101927
6 · The paper itself

Abstract

Age-related macular degeneration (AMD) is a sight threating retinal eye disease that affects millions of aging individuals world-wide. Choroid-retinal pigment epithelium (RPE)-neuroretina axis in the posterior compartment of the eye is the primary site of AMD pathology. There are compelling evidence to indicate association of vascular endothelial growth factors (VEGF) to AMD. Here, we report the inhibitory actions of resveratrol (RSV) on inflammatory cytokine, TGF-β and hypoxia induced VEGF secretion by human retinal pigment epithelial cells (HRPE). HRPE cultures prepared from aged human donor eyes were used for the studies in this report. HRPE secreted both VEGF-A and VEGF-C in small quantities constitutively. Stimulation with a mixture of inflammatory cytokines (IFN-γ, TNF-α, IL-1β), significantly increased the secretion of both VEGF-A and VEGF-C. RSV, in a dose dependent (10-50 uM) manner, suppressed VEGF-A and VEGF-C secretion induced by inflammatory cytokines significantly. RT-PCR analysis indicated that effects of RSV on VEGF secretion were possibly due to decreased mRNA levels. TGF-β and cobalt chloride (hypoxia mimic) also upregulated HRPE cell production of VEGF-A, and this was inhibited by RSV. In contrast, RSV had no effect on anti-angiogenic molecules, endostatin and pigment epithelial derived factor secretion. Studies using an in vitro scratch assay revealed that wound closure was also inhibited by RSV. These results demonstrate that RSV can suppress VEGF secretion induced by inflammatory cytokines, TGF-β and hypoxia. Under pathological conditions, over expression of VEGF is known to worsen AMD. Therefore, RSV may be useful as nutraceutical in controlling pathological choroidal neovascularization processes in AMD.

Indexed as

Age-related macular degenerationCytokinesResveratrolRetinaRetinal pigment epitheliumSIRT1VEGF

Identifiers

PMID24729934
PMCPMC3966676
OpenAlexW1793293664

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.