ArticleJournal of analytical methods in chemistry2014
Simultaneous determination of cortisol and cortisone from human serum by liquid chromatography-tandem mass spectrometry.
Article in Journal of analytical methods in chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- The contribution of serum cortisone and glucocorticoid metabolites to detrimental bone health in patients receiving hydrocortisone therapy.BMC endocrine disorders · 2020Trial
- Flexible ACEK-Enhanced Capacitive Aptasensor for Rapid Cortisol Detection in Sweat.Micromachines · 2026Article
- Determination of hydrocortisone and cortisone in artificial saliva by spray assisted fine droplet formation liquid phase microextraction coupled to LC-MS/MS.Scientific reports · 2026Article
- Highly Responsive Bioassay for Quantification of Glucocorticoids.Analytical chemistry · 2024Article
- Molecularly Imprinted Polymer-Based Sensor for Electrochemical Detection of Cortisol.Biosensors · 2022Review
- Nanomaterials for Cortisol Sensing.Nanomaterials (Basel, Switzerland) · 2022Review
- Article
- Immunosensor for Assessing the Welfare of Trainee Guide Dogs.Biosensors · 2021Article
- The VScientific reports · 2021Article
- Endogenous Glucocorticoid Metabolism in Bone: Friend or Foe.Frontiers in endocrinology · 2021Review
- Nanoantenna-assisted plasmonic enhancement of IR absorption of vibrational modes of organic molecules.Beilstein journal of nanotechnology · 2017Article
- The activity of 11β-hydroxysteroid dehydrogenase type 2 enzyme and cortisol secretion in patients with adrenal incidentalomas.Endocrine · 2016Article
Corrections and comments
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Authors and funding
11 authors.
Funding
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Abstract
A fast, sensitive, and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was validated and then the levels of cortisol and cortisone from sera of healthy adults were determined by the LC-MS/MS method. One hundred μ L of serum sample was directly extracted by adding 2 mL ethyl acetate, followed by chromatographic separation on a C18 column with a mobile phase consisting of 5 mM ammonium acetate and methanol (25 : 75, v/v). The precision, accuracy, and average recovery of the method were 1.5-5.3%, 95.4-102.5%, and 96.4% for cortisol, and 1.9-6.0%, 89.2-98.8%, and 79.9% for cortisone, respectively. The method was linear from 1.0 to 500.0 ng/mL (r(2) = 0.999) for cortisol and 2.5 to 100.0 ng/mL (r(2) = 0.998) for cortisone. The limits of detection (LOD) and quantification (LOQ) were 0.2 and 1.0 ng/mL for cortisol, and 1.0 and 2.5 ng/mL for cortisone, respectively. The average cortisol concentration (133.9 ± 63.7 ng/mL) of samples collected between 9:00 and 11:00 a.m. was higher approximately 4.4 times than that of cortisone (30.5 ± 10.7 ng/mL) (P < 0.0001). The average cortisone/cortisol ratio was 0.225. Therefore, the LC-MS/MS method may be useful for the diagnosis of some adrenal diseases and the assessment of 11 β -hydroxysteroid dehydrogenase (11 β -HSD) activity in clinical laboratories.
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