ArticleSmall (Weinheim an der Bergstrasse, Germany)2014
Facile method for the site-specific, covalent attachment of full-length IgG onto nanoparticles.
Article in Small (Weinheim an der Bergstrasse, Germany), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Antibody Conjugation Strategies for Ultrasound Microbubbles: A Review of Bioconjugation Chemistry and Clinical Translation.Ultrasound in medicine & biology · 2026Review
- Nanosystems Containing Proteins: From Fundamentals to Applications.Chemistry, an Asian journal · 2026Review
- Click Chemistry-Based Bioconjugation of Iron Oxide Nanoparticles.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Nanoparticle Targeting with Antibodies in the Central Nervous System.BME frontiers · 2023Review
- Protein A-Nanoluciferase fusion protein for generalized, sensitive detection of immunoglobulin G.Analytical biochemistry · 2023Article
- Rapid, site-specific labeling of "off-the-shelf" and native serum autoantibodies with T cell-redirecting domains.Science advances · 2022Article
- Rapid Production of Bispecific Antibodies from Off-the-Shelf IgGs with High Yield and Purity.Bioconjugate chemistry · 2022Article
- Affinity-Based Methods for Site-Specific Conjugation of Antibodies.Bioconjugate chemistry · 2021Review
- Universal endogenous antibody recruiting nanobodies capable of triggering immune effectors for targeted cancer immunotherapy.Chemical science · 2021Article
- Targeting cartilage EGFR pathway for osteoarthritis treatment.Science translational medicine · 2021Article
- Dextran-Coated Iron Oxide Nanoparticles as Biomimetic Catalysts for Localized and pH-Activated Biofilm Disruption.ACS nano · 2019Article
- Improving theranostics in pancreatic cancer.Journal of surgical oncology · 2017Review
- Site-selective orientated immobilization of antibodies and conjugates for immunodiagnostics development.Methods (San Diego, Calif.) · 2017Review
- Advances in nano-based inner ear delivery systems for the treatment of sensorineural hearing loss.Advanced drug delivery reviews · 2017Review
- Field Guide to Challenges and Opportunities in Antibody-Drug Conjugates for Chemists.Bioconjugate chemistry · 2015Review
- Click Addition of a DNA Thread to the N-Termini of Peptides for Their Translocation through Solid-State Nanopores.ACS nano · 2015Article
- LASIC: Light Activated Site-Specific Conjugation of Native IgGs.Bioconjugate chemistry · 2015Article
- Superparamagnetic iron oxide nanoparticle micelles stabilized by recombinant oleosin for targeted magnetic resonance imaging.Small (Weinheim an der Bergstrasse, Germany) · 2015Article
- Nanogel Carrier Design for Targeted Drug Delivery.Journal of materials chemistry. B · 2014Article
- Optimization of photoactive protein Z for fast and efficient site-specific conjugation of native IgG.Bioconjugate chemistry · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Antibodies, most commonly IgGs, have been widely used as targeting ligands in research and therapeutic applications due to their wide array of targets, high specificity and proven efficacy. Many of these applications require antibodies to be conjugated onto surfaces (e.g. nanoparticles and microplates); however, most conventional bioconjugation techniques exhibit low crosslinking efficiencies, reduced functionality due to non-site-specific labeling and random surface orientation, and/or require protein engineering (e.g. cysteine handles), which can be technically challenging. To overcome these limitations, we have recombinantly expressed Protein Z, which binds the Fc region of IgG, with an UV active non-natural amino acid benzoylphenyalanine (BPA) within its binding domain. Upon exposure to long wavelength UV light, the BPA is activated and forms a covalent link between the Protein Z and the bound Fc region of IgG. This technology was combined with expressed protein ligation (EPL), which allowed for the introduction of a fluorophore and click chemistry-compatible azide group onto the C-terminus of Protein Z during the recombinant protein purification step. This enabled the crosslinked-Protein Z-IgG complexes to be efficiently and site-specifically attached to aza-dibenzocyclooctyne-modified nanoparticles, via copper-free click chemistry.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.