ArticleNucleic acids research2014
Telomere length kinetics assay (TELKA) sorts the telomere length maintenance (tlm) mutants into functional groups.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 15 citations in OpenAlex.
- Article
- A comparative analysis of telomere length maintenance circuits in fission and budding yeast.Frontiers in genetics · 2022Article
- Inactivation of folylpolyglutamate synthetase Met7 results in genome instability driven by an increased dUTP/dTTP ratio.Nucleic acids research · 2020Article
- Article
- Rnr1's role in telomere elongation cannot be replaced by Rnr3: a role beyond dNTPs?Current genetics · 2018Review
- The evolutionarily conserved factor Sus1/ENY2 plays a role in telomere length maintenance.Current genetics · 2018Article
- Mec1Current genetics · 2018Article
- Rnr1, but not Rnr3, facilitates the sustained telomerase-dependent elongation of telomeres.PLoS genetics · 2017Article
- Article
- Article
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genome-wide systematic screens in yeast have uncovered a large gene network (the telomere length maintenance network or TLM), encompassing more than 400 genes, which acts coordinatively to maintain telomere length. Identifying the genes was an important first stage; the next challenge is to decipher their mechanism of action and to organize then into functional groups or pathways. Here we present a new telomere-length measuring program, TelQuant, and a novel assay, telomere length kinetics assay, and use them to organize tlm mutants into functional classes. Our results show that a mutant defective for the relatively unknown MET7 gene has the same telomeric kinetics as mutants defective for the ribonucleotide reductase subunit Rnr1, in charge of the limiting step in dNTP synthesis, or for the Ku heterodimer, a well-established telomere complex. We confirm the epistatic relationship between the mutants and show that physical interactions exist between Rnr1 and Met7. We also show that Met7 and the Ku heterodimer affect dNTP formation, and play a role in non-homologous end joining. Thus, our telomere kinetics assay uncovers new functional groups, as well as complex genetic interactions between tlm mutants.
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