ArticleNucleic acids research2014
Pervasive generation of oppositely oriented spacers during CRISPR adaptation.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 69 citations in OpenAlex.
- Identification of PAM Requirements for thebioRxiv : the preprint server for biology · 2025Article
- Article
- Persistence of plasmids targeted by CRISPR interference in bacterial populations.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Article
- Prespacers formed during primed adaptation associate with the Cas1-Cas2 adaptation complex and the Cas3 interference nuclease-helicase.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Cooperation between Different CRISPR-Cas Types Enables Adaptation in an RNA-Targeting System.mBio · 2021Article
- Reproducible Antigen Recognition by the Type I-F CRISPR-Cas System.The CRISPR journal · 2020Article
- Real-time observation of CRISPR spacer acquisition by Cas1-Cas2 integrase.Nature structural & molecular biology · 2020Article
- Processing and integration of functionally oriented prespacers in theThe Journal of biological chemistry · 2020Article
- Article
- Detection of CRISPR adaptation.Biochemical Society transactions · 2020Review
- Fidelity of prespacer capture and processing is governed by the PAM-mediated interactions of Cas1-2 adaptation complex in CRISPR-Cas type I-E system.The Journal of biological chemistry · 2019Article
- Article
- Detection of spacer precursors formed in vivo during primed CRISPR adaptation.Nature communications · 2019Article
- Systematic analysis of Type I-E Escherichia coli CRISPR-Cas PAM sequences ability to promote interference and primed adaptation.Molecular microbiology · 2019Article
- CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool.Antibiotics (Basel, Switzerland) · 2019Review
- Mechanisms of Type I-E and I-F CRISPR-Cas Systems inEcoSal Plus · 2019Review
- Article
- Role of nucleotide identity in effective CRISPR target escape mutations.Nucleic acids research · 2018Article
- Cas4 Nucleases Define the PAM, Length, and Orientation of DNA Fragments Integrated at CRISPR Loci.Molecular cell · 2018Article
Corrections and comments
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Authors and funding
6 authors at 6 institutions in 2 countries.
Funding
Abstract
During the process of prokaryotic CRISPR adaptation, a copy of a segment of foreign deoxyribonucleic acid referred to as protospacer is added to the CRISPR cassette and becomes a spacer. When a protospacer contains a neighboring target interference motif, the specific small CRISPR ribonucleic acid (crRNA) transcribed from expanded CRISPR cassette can protect a prokaryotic cell from virus infection or plasmid transformation and conjugation. We show that in Escherichia coli, a vast majority of plasmid protospacers generate spacers integrated in CRISPR cassette in two opposing orientations, leading to frequent appearance of complementary spacer pairs in a population of cells that underwent CRISPR adaptation. When a protospacer contains a spacer acquisition motif AAG, spacer orientation that generates functional protective crRNA is strongly preferred. All other protospacers give rise to spacers oriented in both ways at comparable frequencies. This phenomenon increases the repertoire of available spacers and should make it more likely that a protective crRNA is formed as a result of CRISPR adaptation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.