ArticleNucleic acids research2014
Synthetic circuit for exact adaptation and fold-change detection.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Synthetic dynamic transcription frameworks and their applications.Nature chemistry · 2026Review
- Dynamic Control of DNA Origami Self-Assembly by Transcriptional Modules.Journal of the American Chemical Society · 2026Article
- A Modular T7 RNA Polymerase Toolbox Linking Selective miRNA Detection to Signal Amplification and Protein Expression.Journal of the American Chemical Society · 2025Article
- Supervised learning in DNA neural networks.Nature · 2025Article
- Article
- Y-switch: a spring-loaded synthetic gene switch for robust DNA/RNA signal amplification and detection.Nucleic acids research · 2024Article
- Strategies to Reduce Promoter-Independent Transcription of DNA Nanostructures and Strand Displacement Complexes.ACS synthetic biology · 2024Review
- DNA-empowered synthetic cells as minimalistic life forms.Nature reviews. Chemistry · 2024Review
- Incoherent merger network for robust ratiometric gene expression response.Nucleic acids research · 2023Article
- Dynamic Transcription Machineries Guide the Synthesis of Temporally Operating DNAzymes, Gated and Cascaded DNAzyme Catalysis.ACS nano · 2023Article
- Standardized excitable elements for scalable engineering of far-from-equilibrium chemical networks.Nature chemistry · 2022Article
- Vertex results for the robust analysis of uncertain biochemical systems.Journal of mathematical biology · 2022Article
- Cascaded dissipative DNAzyme-driven layered networks guide transient replication of coded-strands as gene models.Nature communications · 2022Article
- Model-Based Investigation of the Relationship between Regulation Level and Pulse Property of I1-FFL Gene Circuits.ACS synthetic biology · 2022Article
- Design and Evaluation of Synthetic RNA-Based Incoherent Feed-Forward Loop Circuits.Biomolecules · 2021Article
- Autonomous and Assisted Control for Synthetic Microbiology.International journal of molecular sciences · 2020Review
- Experimental evidence for constraints in amplitude-timescale co-variation of a biomolecular pulse generating circuit design.IET systems biology · 2020Article
- Building in vitro transcriptional regulatory networks by successively integrating multiple functional circuit modules.Nature chemistry · 2019Article
- PERSIA for Direct Fluorescence Measurements of Transcription, Translation, and Enzyme Activity in Cell-Free Systems.ACS synthetic biology · 2019Article
- Cell-Free Synthetic Biology Platform for Engineering Synthetic Biological Circuits and Systems.Methods and protocols · 2019Review
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biological organisms use their sensory systems to detect changes in their environment. The ability of sensory systems to adapt to static inputs allows wide dynamic range as well as sensitivity to input changes including fold-change detection, a response that depends only on fold changes in input, and not on absolute changes. This input scale invariance underlies an important strategy for search that depends solely on the spatial profile of the input. Synthetic efforts to reproduce the architecture and response of cellular circuits provide an important step to foster understanding at the molecular level. We report the bottom-up assembly of biochemical systems that show exact adaptation and fold-change detection. Using a malachite green aptamer as the output, a synthetic transcriptional circuit with the connectivity of an incoherent feed-forward loop motif exhibits pulse generation and exact adaptation. A simple mathematical model was used to assess the amplitude and duration of pulse response as well as the parameter regimes required for fold-change detection. Upon parameter tuning, this synthetic circuit exhibits fold-change detection for four successive rounds of two-fold input changes. The experimental realization of fold-change detection circuit highlights the programmability of transcriptional switches and the ability to obtain predictive dynamical systems in a cell-free environment for technological applications.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.