ArticleCell and tissue research1989
Origin of the met-enkephalinergic innervation of the lateral septum in the rat.
Article in Cell and tissue research, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 37 citations in OpenAlex.
- A developmentally defined population of neurons in the lateral septum controls responses to aversive stimuli.bioRxiv : the preprint server for biology · 2023Article
- Top-down regulation of motivated behaviors via lateral septum sub-circuits.Molecular psychiatry · 2022Review
- A Newly Defined Area of the Mouse Anterior Hypothalamus Involved in Septohypothalamic Circuit: Perifornical Area of the Anterior Hypothalamus, PeFAH.Acta histochemica et cytochemica · 2018Article
- Identification of urocortin 3 afferent projection to the ventromedial nucleus of the hypothalamus in rat brain.The Journal of comparative neurology · 2011Article
- Identification of urocortin III, an additional member of the corticotropin-releasing factor (CRF) family with high affinity for the CRF2 receptor.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Light- and electron-microscopic study of electrophysiologically characterized neurons in the mediolateral part of the lateral spetum of the guinea-pig.Cell and tissue research · 1994Article
- Morphological analysis of the neurons in the area of the hypothalamic magnocellular dorsal nucleus of the guinea pig.Cell and tissue research · 1992Article
- Protein-gold complexes as neuronal markers for long-term tracing studies.Histochemistry · 1989Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The location of the cells giving rise to the methionine-enkephalin (Met-Enk)-ergic innervation of the lateral septal nucleus has been investigated in the rat by combining immunohistochemistry and retrograde axonal tracing. Small volumes (0.06 microliter) of apo-horseradish peroxidase (Apo-HRP) conjugated to wheat-germ agglutinin (WGA) and coupled with colloidal gold particles (WGA-ApoHRP-gold) were injected into the lateral septum. The retrogradely labeled cell bodies were visualized by silver intensification of the gold particles on Vibratome sections that were subsequently processed for immunohistochemistry for Met-Enk. Cells labeled with WGA-ApoHRP-gold were observed in the septal area, throughout the hypothalamus (mainly in the perifornical and lateral nuclei) and in the mesencephalon. The localization of Met-Enk-immunoreactive cells was as previously described. With the exception of a few septal cells close to the injection site, doubly labeled cells were found only in the perifornical nucleus of the hypothalamus. Almost all perifornical magnocellular cells were doubly labeled ipsilateral to the injection site, whereas on the opposite side, only about 25% of the Met-Enk-immunoreactive cells contained WGA-ApoHRP-gold. Other brain regions containing retrogradely labeled or Met-Enk-immunoreactive cells (particularly the raphe nuclei) did not show double-labeled neurons. This study demonstrates, using a new and sensitive technique for specific neurochemical tracing of tracts, that the origin of the Met-Enk-ergic innervation of the rat lateral septal nuclei lies in the magnocellular perifornical nuclei of the hypothalamus. The precise involvement of this pathway in limbic functions remains to be determined.
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