ArticleGenome biology and evolution2014
Evolution of bacterial protein-tyrosine kinases and their relaxed specificity toward substrates.
Article in Genome biology and evolution, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
- Identification of EcpK, a bacterial tyrosine pseudokinase important for exopolysaccharide biosynthesis inJournal of bacteriology · 2025Article
- Connection between protein-tyrosine kinase inhibition and coping with oxidative stress inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Comparative Phenotypic, Proteomic, and Phosphoproteomic Analysis Reveals Different Roles of Serine/Threonine Phosphatase and Kinase in the Growth, Cell Division, and Pathogenicity ofMicroorganisms · 2021Article
- Antibacterial Profile of a Microbicidal Agent Targeting Tyrosine Phosphatases and Redox Thiols, Novel Drug Targets.Antibiotics (Basel, Switzerland) · 2021Article
- An ATPase with a twist: A unique mechanism underlies the activity of the bacterial tyrosine kinase, Wzc.Science advances · 2021Article
- A Conserved Structural Role for the Walker-A Lysine in P-Loop Containing Kinases.Frontiers in molecular biosciences · 2021Article
- Role of diversity-generating retroelements for regulatory pathway tuning in cyanobacteria.BMC genomics · 2020Article
- The composition of cultivable bacteria, bacterial pollution, and environmental variables of the coastal areas: an example from the Southeastern Black Sea, Turkey.Environmental monitoring and assessment · 2020Article
- Tyrosine Phosphorylation as a Widespread Regulatory Mechanism in Prokaryotes.Journal of bacteriology · 2019Review
- Tailor-Made Detection of Individual Phosphorylated and Non-Phosphorylated EPIYA-Motifs ofCancers · 2019Article
- Expanding the Scope of Protein Synthesis Using Modified Ribosomes.Journal of the American Chemical Society · 2019Article
- Article
- Structural Analysis of the Hanks-Type Protein Kinase YabT FromFrontiers in microbiology · 2018Article
- SCMBYK: prediction and characterization of bacterial tyrosine-kinases based on propensity scores of dipeptides.BMC bioinformatics · 2016Article
- NADPH oxidase-derived H2O2 subverts pathogen signaling by oxidative phosphotyrosine conversion to PB-DOPA.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Protein phosphorylation and its role in archaeal signal transduction.FEMS microbiology reviews · 2016Review
- Genome dynamics of multidrug-resistant Acinetobacter baumannii during infection and treatment.Genome medicine · 2016Article
- Tyrosine 601 of Bacillus subtilis DnaK Undergoes Phosphorylation and Is Crucial for Chaperone Activity and Heat Shock Survival.Frontiers in microbiology · 2016Article
- Substrate Specificity of theFrontiers in microbiology · 2016Article
- Protein-tyrosine phosphorylation in Bacillus subtilis: a 10-year retrospective.Frontiers in microbiology · 2015Article
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It has often been speculated that bacterial protein-tyrosine kinases (BY-kinases) evolve rapidly and maintain relaxed substrate specificity to quickly adopt new substrates when evolutionary pressure in that direction arises. Here, we report a phylogenomic and biochemical analysis of BY-kinases, and their relationship to substrates aimed to validate this hypothesis. Our results suggest that BY-kinases are ubiquitously distributed in bacterial phyla and underwent a complex evolutionary history, affected considerably by gene duplications and horizontal gene transfer events. This is consistent with the fact that the BY-kinase sequences represent a high level of substitution saturation and have a higher evolutionary rate compared with other bacterial genes. On the basis of similarity networks, we could classify BY kinases into three main groups with 14 subgroups. Extensive sequence conservation was observed only around the three canonical Walker motifs, whereas unique signatures proposed the functional speciation and diversification within some subgroups. The relationship between BY-kinases and their substrates was analyzed using a ubiquitous substrate (Ugd) and some Firmicute-specific substrates (YvyG and YjoA) from Bacillus subtilis. No evidence of coevolution between kinases and substrates at the sequence level was found. Seven BY-kinases, including well-characterized and previously uncharacterized ones, were used for experimental studies. Most of the tested kinases were able to phosphorylate substrates from B. subtilis (Ugd, YvyG, and YjoA), despite originating from very distant bacteria. Our results are consistent with the hypothesis that BY-kinases have evolved relaxed substrate specificity and are probably maintained as rapidly evolving platforms for adopting new substrates.
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