ArticleCurrent genetics2014
An optimized transformation protocol for Lipomyces starkeyi.
Article in Current genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Carbon source-driven metabolic and regulatory remodeling defines phenomic states in Lipomyces starkeyi.Scientific reports · 2026Article
- Efficient vitamin A production in Lipomyces starkeyi through metabolic engineering of the β-carotene and retinoid pathways.Microbial cell factories · 2026Article
- A CRISPR/Cas9-based genome-editing platform enabling efficient and precise gene replacement in Lipomyces starkeyi.FEMS yeast research · 2026Article
- Enabling malic acid production from corn-stover hydrolysate in Lipomyces starkeyi via metabolic engineering and bioprocess optimization.Microbial cell factories · 2025Article
- Genome-scale model development and genomic sequencing of the oleaginous cladeFrontiers in bioengineering and biotechnology · 2024Article
- System analysis of Lipomyces starkeyi during growth on various plant-based sugars.Applied microbiology and biotechnology · 2022Article
- Recent advances in genetic technology development of oleaginous yeasts.Applied microbiology and biotechnology · 2022Review
- The history, state of the art and future prospects for oleaginous yeast research.Microbial cell factories · 2021Review
- Strains and approaches for genetic crosses in the oleaginous yeast Lipomyces starkeyi.Yeast (Chichester, England) · 2021Article
- Citrate-Mediated Acyl-CoA Synthesis Is Required for the Promotion of Growth and Triacylglycerol Production in Oleaginous YeastMicroorganisms · 2021Article
- A metabolic model of Lipomyces starkeyi for predicting lipogenesis potential from diverse low-cost substrates.Biotechnology for biofuels · 2021Article
- Carotenoid Production in Oleaginous Yeasts.Advances in experimental medicine and biology · 2021Review
- Engineering an oleaginous yeast Candida tropicalis SY005 for enhanced lipid production.Applied microbiology and biotechnology · 2020Article
- Lipid metabolism of the oleaginous yeast Lipomyces starkeyi.Applied microbiology and biotechnology · 2020Review
- Deletion of the KU70 homologue facilitates gene targeting in Lipomyces starkeyi strain NRRL Y-11558.Current genetics · 2019Article
- Lipomyces starkeyi: an emerging cell factory for production of lipids, oleochemicals and biotechnology applications.World journal of microbiology & biotechnology · 2018Review
- Optimization of C16 and C18 fatty alcohol production by an engineered strain of Lipomyces starkeyi.Journal of industrial microbiology & biotechnology · 2018Article
- Expression of an endoglucanase-cellobiohydrolase fusion protein inBiotechnology for biofuels · 2018Article
- Efficient gene targeting in non-homologous end-joining-deficient Lipomyces starkeyi strains.Current genetics · 2017Article
- Expression and secretion of fungal endoglucanase II and chimeric cellobiohydrolase I in the oleaginous yeast Lipomyces starkeyi.Microbial cell factories · 2017Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report the development of an efficient genetic transformation system for Lipomyces starkeyi based on a modified lithium acetate transformation protocol. L. starkeyi is a highly lipogenic yeast that grows on a wide range of substrates. The initial transformation rate for this species was extremely low, and required very high concentrations of DNA. A systematic approach for optimizing the protocol resulted in an increase in the transformation efficiency by four orders of magnitude. Important parameters included cell density, the duration of incubation and recovery periods, the heat shock temperature, and the concentration of lithium acetate and carrier DNA within the transformation mixture. We have achieved efficiencies in excess of 8,000 transformants/µg DNA, which now make it possible to screen libraries in the metabolic engineering of this yeast. Metabolic engineering based on this transformation system could improve lipogenesis and enable formation of higher value products.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.