ArticleThe Plant cell2014
Efficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation Sequencing.
Article in The Plant cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 133 papers.
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Who cites it
133 citing papers in PubMed.
- Comparative Genome-Wide Association Studies of Metabolites and Grain-Related Traits in Common Wheat.Plant biotechnology journal · 2026Article
- Ethyl methanesulfonate mutagenesis: Advancing bacterial genetics for sustainable agriculture.Folia microbiologica · 2026Review
- The annotated blueprint: integrated functional genomic resources for a model tetraploid wheat Triticum turgidum cv Kronos.The New phytologist · 2026Article
- Telomere-to-telomere genome assembly and comprehensive mutation library facilitate bitter gourd breeding and functional genomics research.Plant communications · 2026Article
- Investigation of Physiological Responses of Different Soybean Cultivars Under Drought Stress.Plants (Basel, Switzerland) · 2026Article
- Harnessing Bulk-Segregant Mapping to Identify Trait-Associated Genes in the Allopolyploid Model Plant Nicotiana benthamiana.Plant biotechnology journal · 2026Article
- Spatial variation in the mutation rate within the plant shoot apical meristem.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Genome assembly and DNA methylation variation in an epimutant population of hybrid poplar clone NL895.Plant physiology · 2025Article
- The Annotated Blueprint: Integrated Functional Genomic Resources for a model Tetraploid WheatbioRxiv : the preprint server for biology · 2025Article
- Clonal strains of the fresh-market potato cultivar Russet Norkotah changed the domestication gene CDF1.Plant physiology · 2025Article
- Durum wheat mutants with enhanced disease resistance to stripe rust show differential responses to other fungal diseases.Molecular breeding : new strategies in plant improvement · 2025Article
- Sodium azide mutagenesis induces a unique pattern of mutations.PLoS genetics · 2025Article
- Progress and innovations of gene cloning in wheat and its close relatives.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Review
- Directed mutagenesis in fruit crops.3 Biotech · 2025Review
- Jan and mini-Jan, a model system for potato functional genomics.Plant biotechnology journal · 2025Article
- Creating a gene-indexed EMS mutation library of Zheng58 for improving maize genetics research.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Elevated accumulation of lutein and zeaxanthin in a novel high-biomass yielding strain Dunaliella sp. ZP-1 obtained through EMS mutagenesis.Biotechnology for biofuels and bioproducts · 2025Article
- A TaSnRK1α-TaCAT2 model mediates resistance to Fusarium crown rot by scavenging ROS in common wheat.Nature communications · 2025Article
- Integration of multi-omics data accelerates molecular analysis of common wheat traits.Nature communications · 2025Article
- Article
73 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chemical mutagenesis efficiently generates phenotypic variation in otherwise homogeneous genetic backgrounds, enabling functional analysis of genes. Advances in mutation detection have brought the utility of induced mutant populations on par with those produced by insertional mutagenesis, but systematic cataloguing of mutations would further increase their utility. We examined the suitability of multiplexed global exome capture and sequencing coupled with custom-developed bioinformatics tools to identify mutations in well-characterized mutant populations of rice (Oryza sativa) and wheat (Triticum aestivum). In rice, we identified ∼18,000 induced mutations from 72 independent M2 individuals. Functional evaluation indicated the recovery of potentially deleterious mutations for >2600 genes. We further observed that specific sequence and cytosine methylation patterns surrounding the targeted guanine residues strongly affect their probability to be alkylated by ethyl methanesulfonate. Application of these methods to six independent M2 lines of tetraploid wheat demonstrated that our bioinformatics pipeline is applicable to polyploids. In conclusion, we provide a method for developing large-scale induced mutation resources with relatively small investments that is applicable to resource-poor organisms. Furthermore, our results demonstrate that large libraries of sequenced mutations can be readily generated, providing enhanced opportunities to study gene function and assess the effect of sequence and chromatin context on mutations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.