ArticlePloS one2014
Differentiation of human pluripotent stem cells into nephron progenitor cells in a serum and feeder free system.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 72 citations in OpenAlex.
- Bridging Kidney Organoid Innovation and Regenerative Medicine: Current Advances and Future Directions.Cell proliferation · 2026Review
- Enriched human embryonic stem cells-derived CD133Regenerative therapy · 2024Article
- Human pluripotent stem cell-derived kidney organoids: Current progress and challenges.World journal of stem cells · 2024Review
- Determining epigenetic memory in kidney proximal tubule cell derived induced pluripotent stem cells using a quadruple transgenic reprogrammable mouse.Scientific reports · 2022Article
- A Concise Review on Electrospun Scaffolds for Kidney Tissue Engineering.Bioengineering (Basel, Switzerland) · 2022Review
- Urological cancer organoids, patients' avatars for precision medicine: past, present and future.Cell & bioscience · 2022Review
- Role of extracellular matrix components and structure in new renal modelsFrontiers in physiology · 2022Review
- Concise review: current trends on applications of stem cells in diabetic nephropathy.Cell death & disease · 2020Review
- Kidney Organoids and Tubuloids.Cells · 2020Review
- Differentiating Induced Pluripotent Stem Cells into Renal Cells: A New Approach to Treat Kidney Diseases.Stem cells international · 2020Review
- Recapitulating kidney development in vitro by priming and differentiating mouse embryonic stem cells in monolayers.NPJ Regenerative medicine · 2020Article
- Transplantation of Mouse Induced Pluripotent Stem Cell-Derived Podocytes in a Mouse Model of Membranous Nephropathy Attenuates Proteinuria.Scientific reports · 2019Article
- Recapitulating kidney development: Progress and challenges.Seminars in cell & developmental biology · 2019Review
- Directed Differentiation of Human Pluripotent Stem Cells to Podocytes under Defined Conditions.Scientific reports · 2019Article
- Renogenic characterization and in vitro differentiation of rat amniotic fluid stem cells into renal proximal tubular- and juxtaglomerular-like cells.In vitro cellular & developmental biology. Animal · 2019Article
- Engineering renal epithelial cells: programming and directed differentiation towards glomerular podocyte's progenitor and mature podocyte.American journal of translational research · 2019Review
- Generation of Functioning Nephrons by Implanting Human Pluripotent Stem Cell-Derived Kidney Progenitors.Stem cell reports · 2018Article
- Regenerative Medicine, Disease Modeling, and Drug Discovery in Human Pluripotent Stem Cell-derived Kidney Tissue.European Medical Journal. Reproductive health · 2017Article
- Kidney Organoids: A Translational Journey.Trends in molecular medicine · 2017Review
- Generation of kidney tubular organoids from human pluripotent stem cells.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesKidney disease is emerging as a critical medical problem worldwide. Because of limited treatment options for the damaged kidney, stem cell treatment is becoming an alternative therapeutic approach. Of many possible human stem cell sources, pluripotent stem cells are most attractive due to their self-renewal and pluripotent capacity. However, little is known about the derivation of renal lineage cells from human pluripotent stem cells (hPSCs). In this study, we developed a novel protocol for differentiation of nephron progenitor cells (NPCs) from hPSCs in a serum- and feeder-free system. MATERIALS AND
methodsWe designed step-wise protocols for differentiation of human pluripotent stem cells toward primitive streak, intermediate mesoderm and NPCs by recapitulating normal nephrogenesis. Expression of key marker genes was examined by RT-PCR, real time RT-PCR and immunocytochemistry. Each experiment was independently performed three times to confirm its reproducibility.
resultsAfter modification of culture period and concentration of exogenous factors, hPSCs can differentiate into NPCs that markedly express specific marker genes such as SIX2, GDNF, HOXD11, WT1 and CITED1 in addition to OSR1, PAX2, SALL1 and EYA1. Moreover, NPCs possess the potential of bidirectional differentiation into both renal tubular epithelial cells and glomerular podocytes in defined culture conditions. In particular, approximately 70% of SYN-positive cells were obtained from hPSC-derived NPCs after podocytes induction. NPCs can also form in vitro tubule-like structures in three dimensional culture systems.
conclusionsOur novel protocol for hPSCs differentiation into NPCs can be useful for producing alternative sources of cell replacement therapy and disease modeling for human kidney diseases.
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