ArticleJournal of biomedical optics2014
Excitation-scanning hyperspectral imaging microscope.
Article in Journal of biomedical optics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
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The trial behind it
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Who cites it
48 citing papers in PubMed, 66 citations in OpenAlex.
- Illuminating native fluorescence signatures for cancer detection.Proceedings of SPIE--the International Society for Optical Engineering · 2026Article
- Combined excitation scan-based hyperspectral imaging and digital deconvolution for the assessment of the kinetic and spatial distributions of cAMP signals.Proceedings of SPIE--the International Society for Optical Engineering · 2026Article
- Andrology laboratory techniques for micro-TESE/IVF/ICSI: a narrative review.Asian journal of andrology · 2025Review
- Design of Multiplexed, Live Cell Imaging Experiments Using Excitation Scan-Based Hyperspectral Imaging Microscopy.Proceedings of SPIE--the International Society for Optical Engineering · 2025Article
- A naturally brighter approach to colorectal cancer detection.Proceedings of SPIE--the International Society for Optical Engineering · 2025Article
- Fluorescence excitation-scanning hyperspectral imaging with scalable 2D-3D deep learning framework for colorectal cancer detection.Scientific reports · 2024Article
- Hyperspectral imaging and dynamic region of interest tracking approaches to quantify localized cAMP signals.Biochemical Society transactions · 2024Review
- Development status of novel spectral imaging techniques and application to traditional Chinese medicine.Journal of pharmaceutical analysis · 2023Review
- Comparing Performance of Spectral Image Analysis Approaches for Detection of Cellular Signals in Time-Lapse Hyperspectral Imaging Fluorescence Excitation-Scanning Microscopy.Bioengineering (Basel, Switzerland) · 2023Article
- Multifaceted mirror array illuminator for fluorescence excitation-scanning spectral imaging microscopy.Journal of biomedical optics · 2023Article
- Super-multiplexing excitation spectral microscopy with multiple fluorescence bands.Biomedical optics express · 2022Article
- Microscopy is better in color: development of a streamlined spectral light path for real-time multiplex fluorescence microscopy.Biomedical optics express · 2022Article
- Excitation spectral microscopy for highly multiplexed fluorescence imaging and quantitative biosensing.Light, science & applications · 2021Article
- Excitation-scanning hyperspectral video endoscopy: enhancing the light at the end of the tunnel.Biomedical optics express · 2021Article
- Measurement of 3-Dimensional cAMP Distributions in Living Cells using 4-Dimensional (x, y, z, and λ) Hyperspectral FRET Imaging and Analysis.Journal of visualized experiments : JoVE · 2020Article
- Optical simulations for determining efficacy of new light source designs for excitation-scanning high-speed hyperspectral imaging systems.Proceedings of SPIE--the International Society for Optical Engineering · 2020Article
- Label-free spectroscopic tissue characterization using fluorescence excitation-scanning spectral imaging.Journal of biophotonics · 2020Article
- Comparison of spectral FRET microscopy approaches for single-cell analysis.Proceedings of SPIE--the International Society for Optical Engineering · 2020Article
- Spectral Illumination System Utilizing Spherical Reflection Optics.Proceedings of SPIE--the International Society for Optical Engineering · 2020Article
- Development of a Multicolor Line-Focus Microscope for Rapid Acquisitions of Excitation Spectra.Biophysical journal · 2020Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Hyperspectral imaging is a versatile tool that has recently been applied to a variety of biomedical applications, notably live-cell and whole-tissue signaling. Traditional hyperspectral imaging approaches filter the fluorescence emission over a broad wavelength range while exciting at a single band. However, these emission-scanning approaches have shown reduced sensitivity due to light attenuation from spectral filtering. Consequently, emission scanning has limited applicability for time-sensitive studies and photosensitive applications. In this work, we have developed an excitation-scanning hyperspectral imaging microscope that overcomes these limitations by providing high transmission with short acquisition times. This is achieved by filtering the fluorescence excitation rather than the emission. We tested the efficacy of the excitation-scanning microscope in a side-by-side comparison with emission scanning for detection of green fluorescent protein (GFP)-expressing endothelial cells in highly autofluorescent lung tissue. Excitation scanning provided higher signal-to-noise characteristics, as well as shorter acquisition times (300 ms/wavelength band with excitation scanning versus 3 s/wavelength band with emission scanning). Excitation scanning also provided higher delineation of nuclear and cell borders, and increased identification of GFP regions in highly autofluorescent tissue. These results demonstrate excitation scanning has utility in a wide range of time-dependent and photosensitive applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.