ArticleNature chemical biology2014
Engineering the product profile of a polysialyltransferase.
Article in Nature chemical biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Chemoenzymatic Synthesis of Well-Defined α(2,8)- and α(2,9)-Linked Oligosialosides.Angewandte Chemie (International ed. in English) · 2026Article
- In planta engineering of polysialylated glycoproteins using salmonid polysialyltransferases.Scientific reports · 2026Article
- Carbohydrate-active enzyme (CAZyme) discovery and engineeringRSC chemical biology · 2024Review
- Structural basis for heparan sulfate co-polymerase action by the EXT1-2 complex.Nature chemical biology · 2023Article
- Cellular and Molecular Engineering of Glycan Sialylation in Heterologous Systems.Molecules (Basel, Switzerland) · 2021Review
- An enzyme-based protocol for cell-free synthesis of nature-identical capsular oligosaccharides fromThe Journal of biological chemistry · 2020Article
- Processivity in Bacterial Glycosyltransferases.ACS chemical biology · 2020Review
- Harnessing glycoenzyme engineering for synthesis of bioactive oligosaccharides.Interface focus · 2019Review
- Critical Review of Plant Cell Wall Matrix Polysaccharide Glycosyltransferase Activities Verified by Heterologous Protein Expression.Frontiers in plant science · 2019Review
- A two-phase model for the non-processive biosynthesis of homogalacturonan polysaccharides by the GAUT1:GAUT7 complex.The Journal of biological chemistry · 2018Article
- Shuffling the Neutral Drift of Unspecific Peroxygenase in Saccharomyces cerevisiae.Applied and environmental microbiology · 2018Article
- Efficient solid-phase synthesis of meningococcal capsular oligosaccharides enables simple and fast chemoenzymatic vaccine production.The Journal of biological chemistry · 2018Article
- Polysialylation at Early Stages of Oligodendrocyte Differentiation Promotes Myelin Repair.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- X-ray crystallographic structure of a bacterial polysialyltransferase provides insight into the biosynthesis of capsular polysialic acid.Scientific reports · 2017Article
- Engineering of complex protein sialylation in plants.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Glycosyltransferase engineering for carbohydrate synthesis.Biochemical Society transactions · 2016Review
- Polysialylation controls dendritic cell trafficking by regulating chemokine recognition.Science (New York, N.Y.) · 2016Article
- An efficient cell free enzyme-based total synthesis of a meningococcal vaccine candidate.NPJ vaccines · 2016Article
- The capsule polymerase CslB of Neisseria meningitidis serogroup L catalyzes the synthesis of a complex trimeric repeating unit comprising glycosidic and phosphodiester linkages.The Journal of biological chemistry · 2015Article
- Domain interactions control complex formation and polymerase specificity in the biosynthesis of the Escherichia coli O9a antigen.The Journal of biological chemistry · 2015Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oligo- and polysaccharides have myriad applications as therapeutic reagents from glycoconjugate vaccines to matrices for tissue engineering. Polysaccharide length may vary over several orders of magnitude and is a critical determinant of both their physical properties and biological activities. Therefore, the tailored synthesis of oligo- and polysaccharides of defined size is a major goal for glycoengineering. By mutagenesis and screening of a bacterial polysialyltransferase (polyST), we identified a single-residue switch that controls the size distribution of polymeric products. Specific substitutions at this site yielded distributive enzymes that synthesize polysaccharides with narrow size distribution ideal for glycoengineering applications. Mechanistic investigation revealed that the wild-type enzyme has an extended binding site that accommodates at least 20 residues of the growing polymer; changes in affinity along this binding site allow fine-tuning of the enzyme's product distribution.
Indexed as
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.