ArticleNature methods2014
Single-molecule analysis of cell surface dynamics in Caenorhabditis elegans embryos.
Article in Nature methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
- Nematic structures contribute to robust zygotic polarization in C. elegans.PLoS computational biology · 2026Article
- Rapid actin filament turnover maintains cortical connectivity while allowing for cell cortex deformation and flow.bioRxiv : the preprint server for biology · 2026Article
- Asymmetric distribution of actin-related proteins in the earlybioRxiv : the preprint server for biology · 2026Article
- Actin network heterogeneity tunes activator-inhibitor dynamics at the cell cortex.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Repeated extrinsic and anisotropic mechanical inputs promote C. elegans polarized adherens junction elongation.Developmental cell · 2025Article
- Oligomerization and positive feedback on membrane recruitment encode dynamically stable PAR-3 asymmetries in thebioRxiv : the preprint server for biology · 2025Article
- A minimal mathematical model for polarity establishment and centralspindlin-independent cytokinesis.Journal of cell science · 2025Article
- The secretory pathway in Tetrahymena is organized for efficient constitutive secretion at ciliary pockets.iScience · 2024Article
- C. elegans epicuticlins define specific compartments in the apical extracellular matrix and function in wound repair.Development (Cambridge, England) · 2024Article
- Mechanical and biochemical feedback combine to generate complex contractile oscillations in cytokinesis.Current biology : CB · 2024Article
- Atypical Protein Kinase C Promotes its own Asymmetric Localisation by Phosphorylating Cdc42 in thebioRxiv : the preprint server for biology · 2024Article
- Local monomer levels and established filaments potentiate non-muscle myosin 2 assembly.The Journal of cell biology · 2024Article
- Design principles for selective polarization of PAR proteins by cortical flows.The Journal of cell biology · 2023Article
- Non-invasive chimeric HaloTag labeling to study clustering and diffusion of membrane proteins.STAR protocols · 2022Article
- Oligomerization of peripheral membrane proteins provides tunable control of cell surface polarity.Biophysical journal · 2022Article
- Actomyosin-Driven Division of a Synthetic Cell.ACS synthetic biology · 2022Review
- Rapid assembly of a polar network architecture drives efficient actomyosin contractility.Cell reports · 2022Article
- Modulating RhoA effectors induces transitions to oscillatory and more wavelike RhoA dynamics inMolecular biology of the cell · 2022Article
- A particle size threshold governs diffusion and segregation of PAR-3 during cell polarization.Cell reports · 2022Article
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Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
We describe a general, versatile and minimally invasive method to image single molecules near the cell surface that can be applied to any GFP-tagged protein in Caenorhabditis elegans embryos. We exploited tunable expression via RNAi and a dynamically exchanging monomer pool to achieve fast, continuous single-molecule imaging at optimal densities with signal-to-noise ratios adequate for robust single-particle tracking (SPT). We introduce a method called smPReSS, single-molecule photobleaching relaxation to steady state, that infers exchange rates from quantitative analysis of single-molecule photobleaching kinetics without using SPT. Combining SPT and smPReSS allowed for spatially and temporally resolved measurements of protein mobility and exchange kinetics. We used these methods to (i) resolve distinct mobility states and spatial variation in exchange rates of the polarity protein PAR-6 and (ii) measure spatiotemporal modulation of actin filament assembly and disassembly. These methods offer a promising avenue to investigate dynamic mechanisms that pattern the embryonic cell surface.
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