ArticleThe Journal of biological chemistry2014
Mediator, TATA-binding protein, and RNA polymerase II contribute to low histone occupancy at active gene promoters in yeast.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 30 citations in OpenAlex.
- MEDIATOR SUBUNIT17 integrates jasmonate and auxin signaling pathways to regulate thermomorphogenesis.Plant physiology · 2022Article
- Nutrient sensitive proteinLife science alliance · 2022Article
- Article
- Yeast chromatin remodeling complexes and their roles in transcription.Current genetics · 2020Review
- Article
- Experimental evolution reveals a general role for the methyltransferase Hmt1 in noise buffering.PLoS biology · 2019Article
- Article
- SWI/SNF and RSC cooperate to reposition and evict promoter nucleosomes at highly expressed genes in yeast.Genes & development · 2018Article
- Review
- Transcription regulation by the Mediator complex.Nature reviews. Molecular cell biology · 2018Review
- Proteomic Analysis of the Mediator Complex Interactome in Saccharomyces cerevisiae.Scientific reports · 2017Article
- Tail and Kinase Modules Differently Regulate Core Mediator Recruitment and Function In Vivo.Molecular cell · 2016Article
- Constitutive turnover of histone H2A.Z at yeast promoters requires the preinitiation complex.eLife · 2016Article
- Genome-wide cooperation by HAT Gcn5, remodeler SWI/SNF, and chaperone Ydj1 in promoter nucleosome eviction and transcriptional activation.Genome research · 2016Article
- On the way of revealing coactivator complexes cross-talk during transcriptional activation.Cell & bioscience · 2016Review
- Med14 cooperates with brg1 in the differentiation of skeletogenic neural crest.BMC developmental biology · 2015Article
- A role for epigenetics in the formation of chromosome translocations in acute leukemia.Cancer genetics · 2015Review
- Fungal mediator tail subunits contain classical transcriptional activation domains.Molecular and cellular biology · 2015Article
- The Mediator complex: a central integrator of transcription.Nature reviews. Molecular cell biology · 2015Review
- Genome-wide association of mediator and RNA polymerase II in wild-type and mediator mutant yeast.Molecular and cellular biology · 2015Article
Corrections and comments
- Erratum issued
Authors and funding
16 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcription by RNA polymerase II (Pol II) in eukaryotes requires the Mediator complex, and often involves chromatin remodeling and histone eviction at active promoters. Here we address the role of Mediator in recruitment of the Swi/Snf chromatin remodeling complex and its role, along with components of the preinitiation complex (PIC), in histone eviction at inducible and constitutively active promoters in the budding yeast Saccharomyces cerevisiae. We show that recruitment of the Swi/Snf chromatin remodeling complex to the induced CHA1 promoter, as well as its association with several constitutively active promoters, depends on the Mediator complex but is independent of Mediator at the induced MET2 and MET6 genes. Although transcriptional activation and histone eviction at CHA1 depends on Swi/Snf, Swi/Snf recruitment is not sufficient for histone eviction at the induced CHA1 promoter. Loss of Swi/Snf activity does not affect histone occupancy of several constitutively active promoters; in contrast, higher histone occupancy is seen at these promoters in Mediator and PIC component mutants. We propose that an initial activator-dependent, nucleosome remodeling step allows PIC components to outcompete histones for occupancy of promoter sequences. We also observe reduced promoter association of Mediator and TATA-binding protein in a Pol II (rpb1-1) mutant, indicating mutually cooperative binding of these components of the transcription machinery and indicating that it is the PIC as a whole whose binding results in stable histone eviction.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.