ArticleGlycobiology2014
Immunization with recombinantly expressed glycan antigens from Schistosoma mansoni induces glycan-specific antibodies against the parasite.
Article in Glycobiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 28 citations in OpenAlex.
- Glycan remodelling of live adult Schistosoma mansoni worms by chemical inhibition of α-mannosidases.International journal for parasitology · 2026Article
- Liposomal Co-Delivery of Antigenic Glycan LDNF and α-GalCer Elicits Potent IgG Responses with Potential for Anti-Helminth Immunity.International journal of nanomedicine · 2026Article
- Site- and structure-specific characterization of glycoproteins of H11: potent vaccine candidates against parasitic wormFrontiers in immunology · 2025Article
- Serum antibody screening using glycan arrays.Chemical Society reviews · 2024Review
- Anti-schistosomal immunity to core xylose/fucose in N-glycans.Frontiers in molecular biosciences · 2023Article
- H11-induced immunoprotection is predominantly linked to N-glycan moieties duringFrontiers in immunology · 2022Article
- Novel lamprey antibody recognizes terminal sulfated galactose epitopes on mammalian glycoproteins.Communications biology · 2021Article
- Helminth Glycans at the Host-Parasite Interface and Their Potential for Developing Novel Therapeutics.Frontiers in molecular biosciences · 2021Review
- Exosome-Depleted Excretory-Secretory Products of the Fourth-Stage LarvalFrontiers in immunology · 2021Article
- Biomphalaria glabrata immunity: Post-genome advances.Developmental and comparative immunology · 2020Review
- Development of smart anti-glycan reagents using immunized lampreys.Communications biology · 2020Article
- "Stuck on sugars - how carbohydrates regulate cell adhesion, recognition, and signaling".Glycoconjugate journal · 2019Article
- Isotopic labeling with cellular O-glycome reporter/amplification (ICORA) for comparative O-glycomics of cultured cells.Glycobiology · 2018Article
- Identification of Antigenic Glycans from Schistosoma mansoni by Using a Shotgun Egg Glycan Microarray.Infection and immunity · 2016Article
- Schistosoma mansoni α1,3-fucosyltransferase-F generates the Lewis X antigen.Glycobiology · 2016Article
- Protective immunity against Schistosoma japonicum infection can be provided by IgG antibodies towards periodate-sensitive or periodate-resistant glycans.Parasites & vectors · 2015Article
- Role of siglecs and related glycan-binding proteins in immune responses and immunoregulation.The Journal of allergy and clinical immunology · 2015Review
- Article
- Differential expression of anti-glycan antibodies in schistosome-infected humans, rhesus monkeys and mice.Glycobiology · 2014Article
- Deciphering the glycogenome of schistosomes.Frontiers in genetics · 2014Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Schistosomiasis caused by infection with parasitic helminths of Schistosoma spp. is a major global health problem due to inadequate treatment and lack of a vaccine. The immune response to schistosomes includes glycan antigens, which could be valuable diagnostic markers and vaccine targets. However, no precedent exists for how to design vaccines targeting eukaryotic glycoconjugates. The di- and tri-saccharide motifs LacdiNAc (GalNAcβ1,4GlcNAc; LDN) and fucosylated LacdiNAc (GalNAcβ1,4(Fucα1-3)GlcNAc; LDNF) are the basis for several important schistosome glycan antigens. They occur in monomeric form or as repeating units (poly-LDNF) and as part of a variety of different glycoconjugates. Because chemical synthesis and conjugation of such antigens is exceedingly difficult, we sought to develop a recombinant expression system for parasite glycans. We hypothesized that presentation of parasite glycans on the cell surface would induce glycan-specific antibodies. We generated Chinese hamster ovary (CHO) Lec8 cell lines expressing poly-LDN (L8-GT) and poly-LDNF (L8-GTFT) abundantly on their membrane glycoproteins. Sera from Schistosoma mansoni-infected mice were highly cross-reactive with the cells and with cell-surface N-glycans. Immunizing mice with L8-GT and L8-GTFT cells induced glycan-specific antibodies. The L8-GTFT cells induced a sustained booster response, with antibodies that bound to S. mansoni lysates and recapitulated the exquisite specificity of the anti-parasite response for particular presentations of LDNF antigen. In summary, this recombinant expression system promotes successful generation of antibodies to the glycans of S. mansoni, and it can be adapted to study the role of glycan antigens and anti-glycan immune responses in many other infections and pathologies.
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Registered trials
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