ArticleJournal of bacteriology2014
Arginine deiminase in Staphylococcus epidermidis functions to augment biofilm maturation through pH homeostasis.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
64 citing papers in PubMed, 113 citations in OpenAlex.
- Metabolic flux analysis revealed key roles of ArcB in ADI pathway and IlvC in BCAA biosynthesis duringVirulence · 2026Article
- Hydrochloric Acid Lock Therapy for Catheter-Related Bloodstream Infections: In Vitro Antimicrobial Activity and Catheter Material Compatibility.APMIS : acta pathologica, microbiologica, et immunologica Scandinavica · 2026Article
- Amino acid decarboxylation preservesmBio · 2026Article
- Metabolic and regulatory adaptations of Staphylococcus epidermidis to host stresses.FEMS microbiology reviews · 2026Review
- The Acari Hypothesis, VIII: the human apocrine-mammary-microbiota axis andFrontiers in microbiology · 2026Article
- ArgT functions as an arginine transporter inJournal of bacteriology · 2025Article
- Proteomic Analysis of the Differential Response ofAntibiotics (Basel, Switzerland) · 2025Article
- Review
- Urease promotes pH homeostasis and growth ofJournal of bacteriology · 2025Article
- Prophylactic efficacy of probiotics and their metabolites against Staphylococcus epidermidis.BMC microbiology · 2025Article
- TEAL-Seq: targeted expression analysis sequencing.mSphere · 2025Article
- Regulatory dynamics of arginine metabolism in Staphylococcus aureus.Biochemical Society transactions · 2024Review
- Effects of fluid shear stress on oral biofilm formation and composition and the transcriptional response of Streptococcus gordonii.Molecular oral microbiology · 2024Article
- Diversity of Neurotransmitter-Producing Human Skin Commensals.International journal of molecular sciences · 2024Article
- Bacterial persisters: molecular mechanisms and therapeutic development.Signal transduction and targeted therapy · 2024Review
- Arginine impacts aggregation, biofilm formation, and antibiotic susceptibility inbioRxiv : the preprint server for biology · 2024Article
- The ArgR-Regulated ADI Pathway Facilitates the Survival ofInternational journal of molecular sciences · 2024Article
- Microbiota and metabolic adaptation shapebioRxiv : the preprint server for biology · 2024Article
- An energy-conserving reaction in amino acid metabolism catalyzed by arginine synthetase.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Characterizing arginine, ornithine, and putrescine pathways in enteric pathobionts.MicrobiologyOpen · 2024Article
4 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 2 institutions in 1 country.
Funding
Abstract
Allelic replacement mutants were constructed within arginine deiminase (arcA1 and arcA2) to assess the function of the arginine deiminase (ADI) pathway in organic acid resistance and biofilm formation of Staphylococcus epidermidis 1457. A growth-dependent acidification assay (pH ∼5.0 to ∼5.2) determined that strain 1457 devoid of arginine deiminase activity (1457 ΔADI) was significantly less viable than the wild type following depletion of glucose and in the presence of arginine. However, no difference in viability was noted for individual 1457 ΔarcA1 (native) or ΔarcA2 (arginine catabolic mobile element [ACME]-derived) mutants, suggesting that the native and ACME-derived ADIs are compensatory in S. epidermidis. Furthermore, flow cytometry and electron paramagnetic resonance spectroscopy results suggested that organic acid stress resulted in oxidative stress that could be partially rescued by the iron chelator dipyridyl. Collectively, these results suggest that formation of hydroxyl radicals is partially responsible for cell death via organic acid stress and that ADI-derived ammonia functions to counteract this acid stress. Finally, static biofilm assays determined that viability, ammonia synthesis, and pH were reduced in strain 1457 ΔADI following 120 h of growth in comparison to strain 1457 and the arcA1 and arcA2 single mutants. It is hypothesized that ammonia synthesis via the ADI pathway is important to reduce pH stress in specific microniches that contain high concentrations of organic acids.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.