ArticleJournal of bacteriology2014
Construction and characterization of tetH overexpression and knockout strains of Acidithiobacillus ferrooxidans.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 44 citations in OpenAlex.
- Biofilm engineering through c-di-GMP tuning boosts bioleaching efficiency and arsenic tolerance inApplied and environmental microbiology · 2026Article
- Overexpression of sulfide:quinone reductase (SQR) inApplied and environmental microbiology · 2025Article
- Tetrathionate hydrolase from the acidophilic microorganisms.Frontiers in microbiology · 2024Review
- Review
- Reaction mechanism of tetrathionate hydrolysis based on the crystal structure of tetrathionate hydrolase from Acidithiobacillus ferrooxidans.Protein science : a publication of the Protein Society · 2021Article
- Adaptive mechanism of Acidithiobacillus thiooxidans CCTCC M 2012104 under stress during bioleaching of low-grade chalcopyrite based on physiological and comparative transcriptomic analysis.Journal of industrial microbiology & biotechnology · 2019Article
- Microbially Influenced Corrosion of Stainless Steel by Acidithiobacillus ferrooxidans Supplemented with Pyrite: Importance of Thiosulfate.Applied and environmental microbiology · 2019Article
- Iron and sulfur oxidation pathways of Acidithiobacillus ferrooxidans.World journal of microbiology & biotechnology · 2019Review
- Essential Role of σ Factor RpoF in Flagellar Biosynthesis and Flagella-Mediated Motility ofFrontiers in microbiology · 2019Article
- Transposase-Mediated Chromosomal Integration of Exogenous Genes in Acidithiobacillus ferrooxidans.Applied and environmental microbiology · 2018Article
- In a quest for engineering acidophiles for biomining applications: challenges and opportunities.Genes · 2018Review
- Article
- Multi-omics Reveals the Lifestyle of the Acidophilic, Mineral-Oxidizing Model Species Leptospirillum ferriphilumApplied and environmental microbiology · 2018Article
- Sulfur Oxidation in the Acidophilic AutotrophicFrontiers in microbiology · 2018Review
- The Two-Component System RsrS-RsrR Regulates the Tetrathionate Intermediate Pathway for Thiosulfate Oxidation inFrontiers in microbiology · 2016Article
- Multiple Osmotic Stress Responses inFrontiers in microbiology · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acidithiobacillus ferrooxidans is a major participant in consortia of microorganisms used for bioleaching. It can obtain energy from the oxidation of Fe(2+), H2, S(0), and various reduced inorganic sulfur compounds (RISCs). Tetrathionate is a key intermediate during RISC oxidation, hydrolyzed by tetrathionate hydrolase (TetH), and used as sole energy source. In this study, a tetH knockout (ΔtetH) mutant and a tetH overexpression strain were constructed and characterized. The tetH overexpression strain grew better on sulfur and tetrathionate and possessed a higher rate of tetrathionate utilization and TetH activity than the wild type. However, its cell yields on tetrathionate were much lower than those on sulfur. The ΔtetH mutant could not grow on tetrathionate but could proliferate on sulfur with a lower cell yield than the wild type's, which indicated that tetrathionate hydrolysis is mediated only by TetH, encoded by tetH. The ΔtetH mutant could survive in ferrous medium with an Fe(2+) oxidation rate similar to that of the wild type. For the tetH overexpression strain, the rate was relatively higher than that of the wild type. The reverse transcription-quantitative PCR (qRT-PCR) results showed that tetH and doxD2 acted synergistically, and doxD2 was considered important in thiosulfate metabolism. Of the two sqr genes, AFE_0267 seemed to play as important a role in sulfide oxidation as AFE_1792. This study not only provides a substantial basis for studying the function of the tetH gene but also may serve as a model to clarify other candidate genes involved in sulfur oxidation in this organism.
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