ArticleInternational journal for parasitology2014
Hc-daf-2 encodes an insulin-like receptor kinase in the barber's pole worm, Haemonchus contortus, and restores partial dauer regulation.
Article in International journal for parasitology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 35 citations in OpenAlex.
- Transcriptional profiling and functional characterization of the Hc-NHR-49 gene in ivermectin resistance of Haemonchus contortus.Parasites & vectors · 2026Article
- Host insulin hijacking by a nematode receptor mediates developmental plasticity and sex ratio shifts.Nature communications · 2026Article
- Beyond Pathogenesis: The Nematode Immune Network as the Arbiter of a Host-Virus Truce.Viruses · 2025Review
- Enhancing H11 Protein-Induced Immune Protection AgainstBiology · 2025Article
- Identification and validation of protective glycoproteins inFrontiers in immunology · 2025Article
- Study on the effect of GCY-12 gene on albendazole sensitivity ofFrontiers in veterinary science · 2025Article
- A member of the CAP protein superfamily, Hc-CAP-15, is important for the parasitic-stage development of Haemonchus contortus.Parasites & vectors · 2023Article
- Upregulated galectin-1 in Angiostrongylus cantonensis L5 reduces body fat and increases oxidative stress tolerance.Parasites & vectors · 2022Article
- A novel BR-SMAD is required for larval development in barber's pole wormMicrobial cell (Graz, Austria) · 2020Article
- Identification and characterization of an R-Smad homologue (Hco-DAF-8) from Haemonchus contortus.Parasites & vectors · 2020Article
- A TGF-β type II receptor that associates with developmental transition in Haemonchus contortus in vitro.PLoS neglected tropical diseases · 2019Article
- Profiling microRNAs through development of the parasitic nematode Haemonchus identifies nematode-specific miRNAs that suppress larval development.Scientific reports · 2019Article
- Dauer signalling pathway model for Haemonchus contortus.Parasites & vectors · 2019Article
- Vitellogenins - Yolk Gene Function and Regulation inFrontiers in physiology · 2019Review
- Recent Research Progress in China onFrontiers in microbiology · 2017Review
- Expanding the view on the evolution of the nematode dauer signalling pathways: refinement through gene gain and pathway co-option.BMC genomics · 2016Article
- Reconstruction of the insulin-like signalling pathway of Haemonchus contortus.Parasites & vectors · 2016Article
- Molecular characterization of the Haemonchus contortus phosphoinositide-dependent protein kinase-1 gene (Hc-pdk-1).Parasites & vectors · 2016Article
- The Haemonchus contortus kinome--a resource for fundamental molecular investigations and drug discovery.Parasites & vectors · 2015Article
- Expression of nicotinic acetylcholine receptor subunits from parasitic nematodes in Caenorhabditis elegans.Molecular and biochemical parasitology · 2015Article
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11 authors at 6 institutions in 4 countries.
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Abstract
Infective L3s (iL3s) of parasitic nematodes share common behavioural, morphological and developmental characteristics with the developmentally arrested (dauer) larvae of the free-living nematode Caenorhabditis elegans. It is proposed that similar molecular mechanisms regulate entry into or exit from the dauer stage in C. elegans, and the transition from free-living to parasitic forms of parasitic nematodes. In C. elegans, one of the key factors regulating the dauer transition is the insulin-like receptor (designated Ce-DAF-2) encoded by the gene Ce-daf-2. However, nothing is known about DAF-2 homologues in most parasitic nematodes. Here, using a PCR-based approach, we identified and characterised a gene (Hc-daf-2) and its inferred product (Hc-DAF-2) in Haemonchus contortus (a socioeconomically important parasitic nematode of ruminants). The sequence of Hc-DAF-2 displays significant sequence homology to insulin receptors (IR) in both vertebrates and invertebrates, and contains conserved structural domains. A sequence encoding an important proteolytic motif (RKRR) identified in the predicted peptide sequence of Hc-DAF-2 is consistent with that of the human IR, suggesting that it is involved in the formation of the IR complex. The Hc-daf-2 gene was transcribed in all life stages of H. contortus, with a significant up-regulation in the iL3 compared with other stages. To compare patterns of expression between Hc-daf-2 and Ce-daf-2, reporter constructs fusing the Ce-daf-2 or Hc-daf-2 promoter to sequence encoding GFP were microinjected into the N2 strain of C. elegans, and transgenic lines were established and examined. Both genes showed similar patterns of expression in amphidial (head) neurons, which relate to sensation and signal transduction. Further study by heterologous genetic complementation in a daf-2-deficient strain of C. elegans (CB1370) showed partial rescue of function by Hc-daf-2. Taken together, these findings provide a first insight into the roles of Hc-daf-2/Hc-DAF-2 in the biology and development of H. contortus, particularly in the transition to parasitism.
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