ArticleMolecular oncology2014
Identification of novel non-coding RNA-based negative feedback regulating the expression of the oncogenic transcription factor GLI1.
Article in Molecular oncology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 25 citations in OpenAlex.
- Mechanistic study of antisense long non-coding RNAExploration of targeted anti-tumor therapy · 2026Article
- Natural antisense transcript ofGenes & development · 2021Article
- LncRNA SOX2-OT regulates AKT/ERK and SOX2/GLI-1 expression, hinders therapy, and worsens clinical prognosis in malignant lung diseases.Molecular oncology · 2021Article
- Regulation of GLI1 by cis DNA elements and epigenetic marks.DNA repair · 2019Article
- Review
- Differentiation of Long Non-Coding RNA and mRNA Expression Profiles in Male and FemaleFrontiers in genetics · 2019Article
- Identification of novel GLI1 target genes and regulatory circuits in human cancer cells.Molecular oncology · 2018Article
- Role of GLI Transcription Factors in Pathogenesis and Their Potential as New Therapeutic Targets.International journal of molecular sciences · 2018Review
- The utility of stem cells in pediatric urinary bladder regeneration.Pediatric research · 2018Review
- The Hedgehog-GLI pathway in embryonic development and cancer: implications for pulmonary oncology therapy.Oncotarget · 2017Review
- Long non-coding RNA expression profile in cervical cancer tissues.Oncology letters · 2017Article
- Genome-wide Identification and Characterization of Natural Antisense Transcripts by Strand-specific RNA Sequencing in Ganoderma lucidum.Scientific reports · 2017Article
- Progression of chronic kidney disease: too much cellular talk causes damage.Kidney international · 2017Review
- Histone code and long non-coding RNAs (lncRNAs) aberrations in lung cancer: implications in the therapy response.Clinical epigenetics · 2017Review
- A genome scale RNAi screen identifies GLI1 as a novel gene regulating vorinostat sensitivity.Cell death and differentiation · 2016Article
- Neighboring gene regulation by antisense long non-coding RNAs.International journal of molecular sciences · 2015Review
- Long noncoding RNA profiles identify five distinct molecular subtypes of colorectal cancer with clinical relevance.Molecular oncology · 2014Article
- Identification of novel non-coding RNA-based negative feedback regulating the expression of the oncogenic transcription factor GLI1.Molecular oncology · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
17 authors at 5 institutions in 4 countries.
Funding
Abstract
Non-coding RNAs are a complex class of nucleic acids, with growing evidence supporting regulatory roles in gene expression. Here we identify a non-coding RNA located head-to-head with the gene encoding the Glioma-associated oncogene 1 (GLI1), a transcriptional effector of multiple cancer-associated signaling pathways. The expression of this three-exon GLI1 antisense (GLI1AS) RNA in cancer cells was concordant with GLI1 levels. siRNAs knockdown of GLI1AS up-regulated GLI1 and increased cellular proliferation and tumor growth in a xenograft model system. Conversely, GLI1AS overexpression decreased the levels of GLI1, its target genes PTCH1 and PTCH2, and cellular proliferation. Additionally, we demonstrate that GLI1 knockdown reduced GLI1AS, while GLI1 overexpression increased GLI1AS, supporting the role of GLI1AS as a target gene of the GLI1 transcription factor. Activation of TGFβ and Hedgehog signaling, two known regulators of GLI1 expression, conferred a concordant up-regulation of GLI1 and GLI1AS in cancer cells. Finally, analysis of the mechanism underlying the interplay between GLI1 and GLI1AS indicates that the non-coding RNA elicits a local alteration of chromatin structure by increasing the silencing mark H3K27me3 and decreasing the recruitment of RNA polymerase II to this locus. Taken together, the data demonstrate the existence of a novel non-coding RNA-based negative feedback loop controlling GLI1 levels, thus expanding the repertoire of mechanisms regulating the expression of this oncogenic transcription factor.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.