ArticleNucleic acids research1989
Comparative inhibition of rabbit globin mRNA translation by modified antisense oligodeoxynucleotides.
Article in Nucleic acids research, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 237 citations in OpenAlex.
- From a Concept on a Chalkboard to Gapmer Antisense.ACS omega · 2026Article
- Expanding the toolbox: Emerging antisense oligonucleotide mechanisms for modulating gene expression.Molecular therapy. Nucleic acids · 2026Review
- Antisense oligonucleotide gapmers containing phosphoryl guanidine groups reverse MDR1-mediated multiple drug resistance of tumor cells.Molecular therapy. Nucleic acids · 2022Article
- Antisense Drugs Make Sense for Neurological Diseases.Annual review of pharmacology and toxicology · 2021Review
- Systemic Evaluation of Chimeric LNA/2'-O-Methyl Steric Blockers for Myotonic Dystrophy Type 1 Therapy.Nucleic acid therapeutics · 2020Article
- Bioconjugated Oligonucleotides: Recent Developments and Therapeutic Applications.Bioconjugate chemistry · 2019Review
- Antisense techniques provide robust decrease in GnRH receptor expression with minimal cytotoxicity in GT1-7 cells.Systems biology in reproductive medicine · 2018Article
- Improved Performance of Anti-miRNA Oligonucleotides Using a Novel Non-Nucleotide Modifier.Molecular therapy. Nucleic acids · 2013Article
- Automated solid-phase synthesis of RNA oligonucleotides containing a nonbridging phosphorodithioate linkage via phosphorothioamidites.The Journal of organic chemistry · 2012Article
- Antisense phosphorodiamidate morpholino oligomer length and target position effects on gene-specific inhibition in Escherichia coli.Antimicrobial agents and chemotherapy · 2005Article
- Selection of antisense oligodeoxynucleotides against glutathione S-transferase Mu.RNA (New York, N.Y.) · 2002Article
- Molecular requirements for degradation of a modified sense RNA strand by Escherichia coli ribonuclease H1.Nucleic acids research · 2002Article
- Review
- Identification of a key target sequence to block human immunodeficiency virus type 1 replication within the gag-pol transframe domain.Journal of virology · 2000Article
- Vascular smooth muscle cell proliferation and regrowth after mechanical injury in vitro are Egr-1/NGFI-A-dependent.The American journal of pathology · 1999Article
- Early growth response factor-1 induction by injury is triggered by release and paracrine activation by fibroblast growth factor-2.The American journal of pathology · 1999Article
- DNA antisense strategies in the study of receptors for vasoactive peptides, and of growth and wound-healing factors.Molecular and cellular biochemistry · 1997Review
- Inhibition of ubiquitous mitochondrial creatine kinase expression in HeLa cells by an antisense oligodeoxynucleotide.Molecular and cellular biochemistry · 1997Article
- Antisense oligonucleotide inhibition of hepatitis C virus gene expression in transformed hepatocytes.Journal of virology · 1996Article
- Cloning of mouse integrin alphaV cDNA and role of the alphaV-related matrix receptors in metanephric development.The Journal of cell biology · 1996Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have studied the translation of rabbit globin mRNA in cell free systems (reticulocyte lysate and wheat germ extract) and in microinjected Xenopus oocytes in the presence of anti-sense oligodeoxynucleotides. Results obtained with the unmodified all-oxygen compounds were compared with those obtained when phosphorothioate or alpha-DNA was used. In the wheat germ system a 17-mer sequence targeted to the coding region of beta-globin mRNA was specifically inhibitory when either the unmodified phosphodiester oligonucleotide or its phosphorothioate analogue were used. In contrast no effect was observed with the alpha-oligomer. These results were ascribed to the fact that phosphorothioate oligomers elicit an RNase-H activity comparable to the all-oxygen congeners, while alpha-DNA/mRNA hybrids were a poor substrate. Microinjected Xenopus oocytes followed a similar pattern. The phosphorothioate oligomer was more efficient to prevent translation than the unmodified 17-mer. Inhibition of beta-globin synthesis was observed in the nanomolar concentration range. This result can be ascribed to the nuclease resistance of phosphorothioates as compared to natural phosphodiester linkages, alpha-oligomers were devoid of any inhibitory effect up to 30 microM. Phosphorothioate oligodeoxyribonucleotides were shown to be non-specific inhibitors of protein translation, at concentrations in the micromolar range, in both cell-free systems and oocytes. Non-specific inhibition of translation was dependent on the length of the phosphorothioate oligomer. These non-specific effects were not observed with the unmodified or the alpha-oligonucleotides.
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