ArticleJournal of cellular and molecular medicine2014
Cytosolic chloride ion is a key factor in lysosomal acidification and function of autophagy in human gastric cancer cell.
Article in Journal of cellular and molecular medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 51 citations in OpenAlex.
- Optogenetic regulation of chloride ions in reactive astrocytes may mitigate Parkinson's disease pathology.Scientific reports · 2025Article
- A genetically-encoded fluorescence-based reporter to spatiotemporally investigate mannose-6-phosphate pathway.Molecular biology of the cell · 2024Article
- Physiological roles of chloride ions in bodily and cellular functions.The journal of physiological sciences : JPS · 2023Review
- Role of innate immunity and systemic inflammation in cystic fibrosis disease progression.Heliyon · 2023Review
- Platinum-copper alloy nanoparticles armored with chloride ion transporter to promote electro-driven tumor inhibition.Bioactive materials · 2022Article
- Role of Lysosomal Acidification Dysfunction in Mesenchymal Stem Cell Senescence.Frontiers in cell and developmental biology · 2022Review
- Maternal Overnutrition During Gestation in Sheep Alters Autophagy Associated Pathways in Offspring Heart.Frontiers in genetics · 2021Article
- Moxibustion Improves Chronic Heart Failure by Inhibiting Autophagy and Inflammation via Upregulation of mTOR Expression.Evidence-based complementary and alternative medicine : eCAM · 2021Article
- High Expression ofJournal of clinical medicine · 2019Article
- Article
- Investigating the Influence of Steric Hindrance on Selective Anion Transport.Molecules (Basel, Switzerland) · 2019Article
- An ATP-Regulated Ion Transport Nanosystem for Homeostatic Perturbation Therapy and Sensitizing Photodynamic Therapy by Autophagy Inhibition of Tumors.ACS central science · 2019Article
- Carbocisteine stimulated an increase in ciliary bend angle via a decrease in [ClPflugers Archiv : European journal of physiology · 2019Article
- A lysosomal chloride ion-selective fluorescent probe for biological applications.Chemical science · 2019Article
- The Proposal of Molecular Mechanisms of Weak Organic Acids Intake-Induced Improvement of Insulin Resistance in Diabetes Mellitus via Elevation of Interstitial Fluid pH.International journal of molecular sciences · 2018Review
- Measurement of [ClThe journal of physiological sciences : JPS · 2018Article
- Autophagy and inflammation.Clinical and translational medicine · 2017Review
- Chloride Channels are Involved in the Development of Atrial Fibrillation - A Transcriptomic and proteomic Study.Scientific reports · 2017Article
- Indole-based perenosins as highly potent HCl transporters and potential anti-cancer agents.Scientific reports · 2017Article
- The Mechanistic Links between Insulin and Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) ClInternational journal of molecular sciences · 2017Review
Corrections and comments
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Authors and funding
5 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The purpose of the present study was to clarify roles of cytosolic chloride ion (Cl(-) ) in regulation of lysosomal acidification [intra-lysosomal pH (pHlys )] and autophagy function in human gastric cancer cell line (MKN28). The MKN28 cells cultured under a low Cl(-) condition elevated pHlys and reduced the intra-lysosomal Cl(-) concentration ([Cl(-) ]lys ) via reduction of cytosolic Cl(-) concentration ([Cl(-) ]c ), showing abnormal accumulation of LC3II and p62 participating in autophagy function (dysfunction of autophagy) accompanied by inhibition of cell proliferation via G0 /G1 arrest without induction of apoptosis. We also studied effects of direct modification of H(+) transport on lysosomal acidification and autophagy. Application of bafilomycin A1 (an inhibitor of V-type H(+) -ATPase) or ethyl isopropyl amiloride [EIPA; an inhibitor of Na(+) /H(+) exchanger (NHE)] elevated pHlys and decreased [Cl(-) ]lys associated with inhibition of cell proliferation via induction of G0 /G1 arrest similar to the culture under a low Cl(-) condition. However, unlike low Cl(-) condition, application of the compound, bafilomycin A1 or EIPA, induced apoptosis associated with increases in caspase 3 and 9 without large reduction in [Cl(-) ]c compared with low Cl(-) condition. These observations suggest that the lowered [Cl(-) ]c primarily causes dysfunction of autophagy without apoptosis via dysfunction of lysosome induced by disturbance of intra-lysosomal acidification. This is the first study showing that cytosolic Cl(-) is a key factor of lysosome acidification and autophagy.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.