ArticleBMC genomics2014
Proteomic comparison of Ralstonia solanacearum strains reveals temperature dependent virulence factors.
Article in BMC genomics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- The Role of Heat Shock Protein (Hsp) Chaperones in Environmental Stress Adaptation and Virulence of Plant Pathogenic Bacteria.International journal of molecular sciences · 2025Review
- Phage selection drives resistance-virulence trade-offs inEvolution letters · 2024Article
- Melanin Induction Restores the Pathogenicity ofJournal of fungi (Basel, Switzerland) · 2023Article
- Silicon Controls Bacterial Wilt Disease in Tomato Plants and Inhibits the Virulence-Related Gene Expression ofInternational journal of molecular sciences · 2022Article
- Every cloud has a silver lining: how abiotic stresses affect gene expression in plant-pathogen interactions.Journal of experimental botany · 2021Review
- Implication of the Type III Effector RipS1 in the Cool-Virulence ofFrontiers in plant science · 2021Article
- Acetylome analysis of lysine acetylation in the plant pathogenic bacterium Brenneria nigrifluens.MicrobiologyOpen · 2020Article
- Biocontrol of Bacterial Wilt Disease Through Complex Interaction Between Tomato Plant, Antagonists, the Indigenous Rhizosphere Microbiota, andFrontiers in microbiology · 2019Article
- Comparative Proteomic Analysis of TwoInternational journal of molecular sciences · 2018Article
- Complete genome sequence of the sesame pathogen Ralstonia solanacearum strain SEPPX 05.Genes & genomics · 2018Article
- Article
- Complete Genome Sequences of the Plant PathogensGenome announcements · 2017Article
- Seasonal variation in the biocontrol efficiency of bacterial wilt is driven by temperature-mediated changes in bacterial competitive interactions.The Journal of applied ecology · 2017Article
- Comparative Genomics ofFrontiers in plant science · 2017Article
- Transcriptomes ofFrontiers in plant science · 2017Article
- Global Gene Expression Analysis of Cross-Protected Phenotype of Pectobacterium atrosepticum.PloS one · 2017Article
- Assessing the Pathogenic Ability ofFrontiers in plant science · 2017Article
- Article
- Comparative Transcriptome Analysis Reveals Cool Virulence Factors of Ralstonia solanacearum Race 3 Biovar 2.PloS one · 2015Article
- Altering Transplantation Time to Avoid Periods of High Temperature Can Efficiently Reduce Bacterial Wilt Disease Incidence with Tomato.PloS one · 2015Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRalstonia solanacearum, the causal agent of bacterial wilt, is a genetically diverse bacterial plant pathogen present in tropical and subtropical regions of the world that infects more than 200 plant species, including economically important solanaceous crops. Most strains of R. solanacearum are only pathogenic at temperatures between 25 to 30°C with strains that can cause disease below 20°C considered a threat to agriculture in temperate areas. Identifying key molecular factors that distinguish strains virulent at cold temperatures from ones that are not is needed to develop effective management tools for this pathogen. We compared protein profiles of two strains virulent at low temperature and two strains not virulent at low temperature when incubated in the rhizosphere of tomato seedlings at 30 and 18°C using quantitative 2D DIGE gel methods. Spot intensities were quantified and compared, and differentially expressed proteins were sequenced and identified by mass spectrometry (MS/MS).
resultsFour hundred and eighteen (418) differentially expressed protein spots sequenced produced 101 unique proteins. The identified proteins were classified in the Gene Ontology biological processes categories of metabolism, cell processes, stress response, transport, secretion, motility, and virulence. Identified virulence factors included catalase (KatE), exoglucanase A (ChbA), drug efflux pump, and twitching motility porin (PilQ). Other proteins identified included two components of a putative type VI secretion system. We confirmed differential expression of 13 candidate genes using real time PCR techniques. Global regulators HrpB and HrpG also had temperature dependent expression when quantified by real time PCR.
conclusionsThe putative involvement of the identified proteins in virulence at low temperature is discussed. The discovery of a functional type VI secretion system provides a new potential virulence mechanism to explore. The global regulators HrpG and HrpB, and the protein expression profiles identified suggest that virulence at low temperatures can be partially explained by differences in regulation of virulence factors present in all the strains.
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