ArticleBMC genomics2014
Knockout of an outer membrane protein operon of Anaplasma marginale by transposon mutagenesis.
Article in BMC genomics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 25 citations in OpenAlex.
- A platform supporting generation and isolation of random transposon mutants inJournal of bacteriology · 2025Article
- Development of a Recombinase-Mediated Cassette Exchange System for Gene Knockout and Expression of Non-Native Gene Sequences inVaccines · 2025Article
- Current vaccines, experimental immunization trials, and new perspectives to control selected vector borne blood parasites of veterinary importance.Frontiers in veterinary science · 2024Review
- MultipleFrontiers in cellular and infection microbiology · 2023Article
- Recent advances in genetic systems in obligate intracellular human-pathogenic bacteria.Frontiers in cellular and infection microbiology · 2023Review
- Transposon mutagenesis of Rickettsia felis sca1 confers a distinct phenotype during flea infection.PLoS pathogens · 2022Article
- Bovine Anaplasmosis: Will there ever be an almighty effective vaccine?Frontiers in veterinary science · 2022Review
- Article
- Analysis of Orientia tsutsugamushi promoter activity.Pathogens and disease · 2021Article
- Biostatistical prediction of genes essential for growth of Anaplasma phagocytophilum in a human promyelocytic cell line using a random transposon mutant library.Pathogens and disease · 2021Article
- Disruption of VirB6 Paralogs in Anaplasma phagocytophilum Attenuates Its Growth.Journal of bacteriology · 2020Article
- Multiple Ehrlichia chaffeensis Genes Critical for Its Persistent Infection in a Vertebrate Host Are Identified by Random Mutagenesis Coupled withInfection and immunity · 2020Article
- Sequence Determinants Spanning -10 Motif and Spacer Region Implicated in UniqueFrontiers in microbiology · 2019Article
- A genetic system for targeted mutations to disrupt and restore genes in the obligate bacterium, Ehrlichia chaffeensis.Scientific reports · 2017Article
- Engineering of obligate intracellular bacteria: progress, challenges and paradigms.Nature reviews. Microbiology · 2017Review
- Peptide Nucleic Acid Knockdown and Intra-host Cell Complementation ofFrontiers in cellular and infection microbiology · 2017Article
- Hijacking Host Cell Highways: Manipulation of the Host Actin Cytoskeleton by Obligate Intracellular Bacterial Pathogens.Frontiers in cellular and infection microbiology · 2016Review
- A Coming of Age Story: Chlamydia in the Post-Genetic Era.Infection and immunity · 2015Review
- Reduced Infectivity in cattle for an outer membrane protein mutant of Anaplasma marginale.Applied and environmental microbiology · 2015Article
- An O-Methyltransferase Is Required for Infection of Tick Cells by Anaplasma phagocytophilum.PLoS pathogens · 2015Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundThe large amounts of data generated by genomics, transcriptomics and proteomics have increased our understanding of the biology of Anaplasma marginale. However, these data have also led to new assumptions that require testing, ideally through classical genetic mutation. One example is the definition of genes associated with virulence. Here we describe the molecular characterization of a red fluorescent and spectinomycin and streptomycin resistant A. marginale mutant generated by Himar1 transposon mutagenesis.
resultsHigh throughput genome sequencing to determine the Himar1-A. marginale genome junctions established that the transposon sequences were integrated within the coding region of the omp10 gene. This gene is arranged within an operon with AM1225 at the 5' end and with omp9, omp8, omp7 and omp6 arranged in tandem at the 3' end. RNA analysis to determine the effects of the transposon insertion on the expression of omp10 and downstream genes revealed that the Himar1 insertion not only reduced the expression of omp10 but also that of downstream genes. Transcript expression from omp9, and omp8 dropped by more than 90% in comparison with their counterparts in wild-type A. marginale. Immunoblot analysis showed a reduction in the production of Omp9 protein in these mutants compared to wild-type A. marginale.
conclusionsThese results demonstrate that transposon mutagenesis in A. marginale is possible and that this technology can be used for the creation of insertional gene knockouts that can be evaluated in natural host-vector systems.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.