ArticleBioMed research international2014
Expression of mesenchymal stem cells-related genes and plasticity of aspirated follicular cells obtained from infertile women.
Article in BioMed research international, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 45 citations in OpenAlex.
- Female Age Affects the Mesenchymal Stem Cell Characteristics of Aspirated Follicular Cells in the In Vitro Fertilization Programme.Stem cell reviews and reports · 2019Trial
- Differential Gene Expression of Mesenchymal Stromal Cell and Granulosa Cell Markers in Equine Follicular Aspirates.Reproduction in domestic animals = Zuchthygiene · 2026Article
- Influence of Follicle Number and Reproductive Cyclicity on Mesenchymal Stromal Cell Yield Isolated From Equine Ovarian Follicular Aspirates.Reproduction in domestic animals = Zuchthygiene · 2026Article
- Significant improvement of bone marrow-derived MSC expansion from MDS patients by defined xeno-free medium.Stem cell research & therapy · 2023Article
- Follicle isolation methods reveal plasticity of granulosa cell steroidogenic capacity during mouse in vitro follicle growth.Molecular human reproduction · 2022Article
- The Effect of Different Doses of Melatonin on in Vitro Maturation of Human Follicular Fluid-Derived Oocyte-Like Cells.JBRA assisted reproduction · 2022Article
- Human Granulosa Cells-Stemness Properties, Molecular Cross-Talk and Follicular Angiogenesis.Cells · 2021Review
- Characterization of stem cells from human ovarian follicular fluid; a potential source of autologous stem cell for cell-based therapy.Human cell · 2021Article
- Research Progress on the Treatment of Premature Ovarian Failure Using Mesenchymal Stem Cells: A Literature Review.Frontiers in cell and developmental biology · 2021Review
- Decellularized Cell Culture ECMs Act as Cell Differentiation Inducers.Stem cell reviews and reports · 2020Article
- Expression of genes involved in neurogenesis, and neuronal precursor cell proliferation and development: Novel pathways of human ovarian granulosa cell differentiation and transdifferentiation capability in vitro.Molecular medicine reports · 2020Article
- Tumor necrosis factor α in aGVHD patients contributed to the impairment of recipient bone marrow MSC stemness and deficiency of their hematopoiesis-promotion capacity.Stem cell research & therapy · 2020Article
- Characterization and identification of human immortalized granulosa cells derived from ovarian follicular fluid.Experimental and therapeutic medicine · 2019Article
- Genes responsible for proliferation, differentiation, and junction adhesion are significantly up-regulated in human ovarian granulosa cells during a long-term primary in vitro culture.Histochemistry and cell biology · 2019Article
- Functional Testing of Primitive Oocyte-like Cells Developed in Ovarian Surface Epithelium Cell Culture from Small VSEL-like Stem Cells: Can They Be Fertilized One Day?Stem cell reviews and reports · 2018Article
- FOXP1 enhances fibrosis via activating Wnt/β-catenin signaling pathway in endometriosis.American journal of translational research · 2018Article
- Soluble CD146, an innovative and non-invasive biomarker of embryo selection for in vitro fertilization.PloS one · 2017Article
- Stem cells, progenitor cells, and lineage decisions in the ovary.Endocrine reviews · 2015Review
- Ovarian adult stem cells: hope or pitfall?Journal of ovarian research · 2014Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
After removal of oocytes for in vitro fertilization, follicular aspirates which are rich in somatic follicular cells are discarded in daily medical practice. However, there is some evidence that less differentiated cells with stem cell characteristics are present among aspirated follicular cells (AFCs). The aim of this study was to culture AFCs in vitro and to analyze their gene expression profile. Using the RT2 Profiler PCR array, we investigated the expression profile of 84 genes related to stemness, mesenchymal stem cells (MCSs), and cell differentiation in AFCs enriched by hypoosmotic protocol from follicular aspirates of infertile women involved in assisted reproduction programme in comparison with bone marrow-derived mesenchymal stem cells (BM-MSCs) and fibroblasts. Altogether the expression of 57 genes was detected in AFCs: 16 genes (OCT4, CD49f, CD106, CD146, CD45, CD54, IL10, IL1B, TNF, VEGF, VWF, HDAC1, MITF, RUNX2, PPARG, and PCAF) were upregulated and 20 genes (FGF2, CASP3, CD105, CD13, CD340, CD73, CD90, KDR, PDGFRB, BDNF, COL1A1, IL6, MMP2, NES, NUDT6, BMP6, SMURF2, BMP4, GDF5, and JAG1) were downregulated in AFCs when compared with BM-MSCs. The genes which were upregulated in AFCs were mostly related to MSCs and connected with ovarian function, and differed from those in fibroblasts. The cultured AFCs with predominating granulosa cells were successfully in vitro differentiated into adipogenic-, osteogenic-, and pancreatic-like cells. The upregulation of some MSC-specific genes and in vitro differentiation into other types of cells indicated a subpopulation of AFCs with specific stemness, which was not similar to those of BM-MSCs or fibroblasts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.