ArticleJournal of experimental botany2014
Programmed cell death activated by Rose Bengal in Arabidopsis thaliana cell suspension cultures requires functional chloroplasts.
Article in Journal of experimental botany, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 43 citations in OpenAlex.
- Article
- The multifunctional ascorbate peroxidase MoApx1 secreted by Magnaporthe oryzae mediates the suppression of rice immunity.The Plant cell · 2025Article
- Singlet Oxygen Detection by Chemiluminescence Probes in Living Cells.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Electrical Signals, Plant Tolerance to Actions of Stressors, and Programmed Cell Death: Is Interaction Possible?Plants (Basel, Switzerland) · 2021Review
- Plant Cell Cultures as a Tool to Study Programmed Cell Death.International journal of molecular sciences · 2021Review
- Singlet Oxygen in Plants: Generation, Detection, and Signaling Roles.International journal of molecular sciences · 2020Review
- Cyanobacteria-Derived Proline Increases Stress Tolerance inFrontiers in plant science · 2020Article
- Leaf Senescence: The Chloroplast Connection Comes of Age.Plants (Basel, Switzerland) · 2019Review
- Article
- Dose-dependent effects of 1O2 in chloroplasts are determined by its timing and localization of production.Journal of experimental botany · 2019Review
- The Arabidopsis ATP-BINDING CASSETTE Transporter ABCB21 Regulates Auxin Levels in Cotyledons, the Root Pericycle, and Leaves.Frontiers in plant science · 2019Article
- Noninvasive determination of toxic stress biomarkers by high-throughput screening of photoautotrophic cell suspension cultures with multicolor fluorescence imaging.Plant methods · 2019Article
- A Rapid and Efficient Method to Obtain Photosynthetic Cell Suspension Cultures ofFrontiers in plant science · 2017Article
- Singlet oxygen detection in biological systems: Uses and limitations.Plant signaling & behavior · 2016Article
- Singlet Oxygen-Induced Membrane Disruption and Serpin-Protease Balance in Vacuolar-Driven Cell Death.Plant physiology · 2016Article
- The ROS Wheel: Refining ROS Transcriptional Footprints.Plant physiology · 2016Article
- Singlet Oxygen-Induced Cell Death in Arabidopsis under High-Light Stress Is Controlled by OXI1 Kinase.Plant physiology · 2016Article
- Chloroplast Activity and 3'phosphadenosine 5'phosphate Signaling Regulate Programmed Cell Death in Arabidopsis.Plant physiology · 2016Article
- The effect of UV-B on Arabidopsis leaves depends on light conditions after treatment.BMC plant biology · 2015Article
- Programmed cell death in plants: A chloroplastic connection.Plant signaling & behavior · 2015Review
Corrections and comments
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Authors and funding
8 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Light-grown Arabidopsis thaliana cell suspension culture (ACSC) were subjected to mild photooxidative damage with Rose Bengal (RB) with the aim of gaining a better understanding of singlet oxygen-mediated defence responses in plants. Additionally, ACSC were treated with H2O2 at concentrations that induced comparable levels of protein oxidation damage. Under low to medium light conditions, both RB and H2O2 treatments activated transcriptional defence responses and inhibited photosynthetic activity, but they differed in that programmed cell death (PCD) was only observed in cells treated with RB. When dark-grown ACSC were subjected to RB in the light, PCD was suppressed, indicating that the singlet oxygen-mediated signalling pathway in ACSC requires functional chloroplasts. Analysis of up-regulated transcripts in light-grown ACSC, treated with RB in the light, showed that both singlet oxygen-responsive transcripts and transcripts with a key role in hormone-activated PCD (i.e. ethylene and jasmonic acid) were present. A co-regulation analysis proved that ACSC treated with RB exhibited higher correlation with the conditional fluorescence (flu) mutant than with other singlet oxygen-producing mutants or wild-type plants subjected to high light. However, there was no evidence for the up-regulation of EDS1, suggesting that activation of PCD was not associated with the EXECUTER- and EDS1-dependent signalling pathway described in the flu mutant. Indigo Carmine and Methylene Violet, two photosensitizers unable to enter chloroplasts, did not activate transcriptional defence responses in ACSC; however, whether this was due to their location or to their inherently low singlet oxygen quantum efficiencies was not determined.
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