ArticleElectrophoresis2014
Dielectrophoretic isolation and detection of cancer-related circulating cell-free DNA biomarkers from blood and plasma.
Article in Electrophoresis, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 62 citations in OpenAlex.
- Numerical study on a facing electrode configuration dielectrophoresis microfluidic system for efficient biological cell separation.Scientific reports · 2024Article
- Development of a blood-based extracellular vesicle classifier for detection of early-stage pancreatic ductal adenocarcinoma.Communications medicine · 2023Article
- Dielectrophoresis: An Approach to Increase Sensitivity, Reduce Response Time and to Suppress Nonspecific Binding in Biosensors?Biosensors · 2022Review
- Recent Advances in Device Engineering and Computational Analysis for Characterization of Cell-Released Cancer Biomarkers.Cancers · 2022Review
- Early-stage multi-cancer detection using an extracellular vesicle protein-based blood test.Communications medicine · 2022Article
- A Prominent Cell Manipulation Technique in BioMEMS: Dielectrophoresis.Micromachines · 2020Review
- Scalable Signature-Based Molecular Diagnostics Through On-chip Biomarker Profiling Coupled with Machine Learning.Annals of biomedical engineering · 2020Review
- Direct electrophoretic microRNA preparation from clinical samples using nanofilter membrane.Nano convergence · 2020Article
- Plasma Biomarker for Post-concussive Syndrome: A Pilot Study Using an Alternating Current Electro-Kinetic Platform.Frontiers in neurology · 2020Article
- Thermal Shock Response of Yeast Cells Characterised by Dielectrophoresis Force Measurement.Sensors (Basel, Switzerland) · 2019Article
- Characterization of Simple and Double Yeast Cells Using Dielectrophoretic Force Measurement.Sensors (Basel, Switzerland) · 2019Article
- Recent advances in microfluidic methods in cancer liquid biopsy.Biomicrofluidics · 2019Review
- Dielectrophoresis for Biomedical Sciences Applications: A Review.Sensors (Basel, Switzerland) · 2017Review
- On-chip wavelength multiplexed detection of cancer DNA biomarkers in blood.Biomicrofluidics · 2016Article
- Liquid Biopsies in Oncology and the Current Regulatory Landscape.Molecular diagnosis & therapy · 2016Review
- Application of optically-induced-dielectrophoresis in microfluidic system for purification of circulating tumour cells for gene expression analysis- Cancer cell line model.Scientific reports · 2016Article
- Liquid biopsies for liquid tumors: emerging potential of circulating free nucleic acid evaluation for the management of hematologic malignancies.Cancer biology & medicine · 2016Article
- Dielectrophoretic recovery of DNA from plasma for the identification of chronic lymphocytic leukemia point mutations.International journal of hematologic oncology · 2016Article
- Recovery of Drug Delivery Nanoparticles from Human Plasma Using an Electrokinetic Platform Technology.Small (Weinheim an der Bergstrasse, Germany) · 2015Article
- DNA methylation as a promising landscape: A simple blood test for breast cancer prediction.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2015Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Conventional methods for the isolation of cancer-related circulating cell-free (ccf) DNA from patient blood (plasma) are time consuming and laborious. A DEP approach utilizing a microarray device now allows rapid isolation of ccf-DNA directly from a small volume of unprocessed blood. In this study, the DEP device is used to compare the ccf-DNA isolated directly from whole blood and plasma from 11 chronic lymphocytic leukemia (CLL) patients and one normal individual. Ccf-DNA from both blood and plasma samples was separated into DEP high-field regions, after which cells (blood), proteins, and other biomolecules were removed by a fluidic wash. The concentrated ccf-DNA was detected on-chip by fluorescence, and then eluted for PCR and DNA sequencing. The complete process from blood to PCR required less than 10 min; an additional 15 min was required to obtain plasma from whole blood. Ccf-DNA from the equivalent of 5 μL of CLL blood and 5 μL of plasma was amplified by PCR using Ig heavy-chain variable (IGHV) specific primers to identify the unique IGHV gene expressed by the leukemic B-cell clone. The PCR and DNA sequencing results obtained by DEP from all 11 CLL blood samples and from 8 of the 11 CLL plasma samples were exactly comparable to the DNA sequencing results obtained from genomic DNA isolated from CLL patient leukemic B cells (gold standard).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.