ArticlePloS one2014
De novo transcriptome hybrid assembly and validation in the European earwig (Dermaptera, Forficula auricularia).
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 25 citations in OpenAlex.
- Insect immune systems: same same but different but still same.Frontiers in immunology · 2026Review
- The Molecular Resistance Mechanisms of European Earwigs from Apple Orchards Subjected to Different Management Strategies.Insects · 2023Article
- DNA sequencing in the classroom: complete genome sequence of two earwig (Dermaptera; Insecta) species.Biological research · 2023Article
- The genetic mechanism of selfishness and altruism in parent-offspring coadaptation.Science advances · 2020Article
- IDP-denovo: de novo transcriptome assembly and isoform annotation by hybrid sequencing.Bioinformatics (Oxford, England) · 2018Article
- Article
- Recurrent loss of CenH3 is associated with independent transitions to holocentricity in insects.eLife · 2014Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe European earwig (Forficula auricularia) is an established system for studies of sexual selection, social interactions and the evolution of parental care. Despite its scientific interest, little knowledge exists about the species at the genomic level, limiting the scope of molecular studies and expression analyses of genes of interest. To overcome these limitations, we sequenced and validated the transcriptome of the European earwig. METHODOLOGY AND PRINCIPAL
findingsTo obtain a comprehensive transcriptome, we sequenced mRNA from various tissues and developmental stages of female and male earwigs using Roche 454 pyrosequencing and Illumina HiSeq. The reads were de novo assembled independently and screened for possible microbial contamination and repeated elements. The remaining contigs were combined into a hybrid assembly and clustered to reduce redundancy. A comparison with the eukaryotic core gene dataset indicates that we sequenced a substantial part of the earwig transcriptome with a low level of fragmentation. In addition, a comparative analysis revealed that more than 8,800 contigs of the hybrid assembly show significant similarity to insect-specific proteins and those were assigned for Gene Ontology terms. Finally, we established a quantitative PCR test for expression stability using commonly used housekeeping genes and applied the method to five homologs of known sex-biased genes of the honeybee. The qPCR pilot study confirmed sex specific expression and also revealed significant expression differences between the brain and antenna tissue samples.
conclusionsBy employing two different sequencing approaches and including samples obtained from different tissues, developmental stages, and sexes, we were able to assemble a comprehensive transcriptome of F. auricularia. The transcriptome presented here offers new opportunities to study the molecular bases and evolution of parental care and sociality in arthropods.
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