ArticleInternational journal of molecular sciences2014
Triterpenoid saponin biosynthetic pathway profiling and candidate gene mining of the Ilex asprella root using RNA-Seq.
Article in International journal of molecular sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed.
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- Biologically synthesized CuO nanoparticles induce physiological, metabolic, and molecular changes in the hazel cell cultures.Applied microbiology and biotechnology · 2022Article
- Cytochrome P450 Monooxygenase/Cytochrome P450 Reductase Bi-Enzymatic System Isolated FromFrontiers in plant science · 2022Article
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- Transcriptome and metabolome analysis to reveal major genes of saikosaponin biosynthesis in Bupleurum chinense.BMC genomics · 2021Article
- Differential DNA methylation and gene expression during development of reproductive and vegetative organs in Ilex species.Journal of plant research · 2021Article
- Transcriptome and Metabolome Dynamics Explain Aroma Differences between Green and Red Prickly Ash Fruit.Foods (Basel, Switzerland) · 2021Article
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- Transcriptome Analysis ofInternational journal of molecular sciences · 2020Article
- Susceptibility of virulent and resistantVeterinary world · 2020Article
- Genome assembly provides insights into the genome evolution and flowering regulation of orchardgrass.Plant biotechnology journal · 2020Article
- Dissection of Dynamic Transcriptome Landscape of Leaf, Bract, and Lupulin Gland in Hop (International journal of molecular sciences · 2019Article
- Ursolic Acid and Its Derivatives as Bioactive Agents.Molecules (Basel, Switzerland) · 2019Review
- Identification of potential genes involved in triterpenoid saponins biosynthesis in Gleditsia sinensis by transcriptome and metabolome analyses.Journal of natural medicines · 2019Article
- Deep sequencing and transcriptome analyses to identify genes involved in secoiridoid biosynthesis in the Tibetan medicinal plant Swertia mussotii.Scientific reports · 2017Article
- Positive selection and functional divergence of farnesyl pyrophosphate synthase genes in plants.BMC molecular biology · 2017Article
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5 authors.
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Abstract
Ilex asprella, which contains abundant α-amyrin type triterpenoid saponins, is an anti-influenza herbal drug widely used in south China. In this work, we first analysed the transcriptome of the I. asprella root using RNA-Seq, which provided a dataset for functional gene mining. mRNA was isolated from the total RNA of the I. asprella root and reverse-transcribed into cDNA. Then, the cDNA library was sequenced using an Illumina HiSeq™ 2000, which generated 55,028,452 clean reads. De novo assembly of these reads generated 51,865 unigenes, in which 39,269 unigenes were annotated (75.71% yield). According to the structures of the triterpenoid saponins of I. asprella, a putative biosynthetic pathway downstream of 2,3-oxidosqualene was proposed and candidate unigenes in the transcriptome data that were potentially involved in the pathway were screened using homology-based BLAST and phylogenetic analysis. Further amplification and functional analysis of these putative unigenes will provide insight into the biosynthesis of Ilex triterpenoid saponins.
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