ArticlePLoS genetics2014
Uncoupling transcription from covalent histone modification.
Article in PLoS genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 45 citations in OpenAlex.
- Article
- Heat Shock Factor 1 forms nuclear condensates and restructures the yeast genome before activating target genes.bioRxiv : the preprint server for biology · 2024Article
- Article
- Phase-separation antagonists potently inhibit transcription and broadly increase nucleosome density.The Journal of biological chemistry · 2022Article
- Histone exchange is associated with activator function at transcribed promoters and with repression at histone loci.Science advances · 2020Article
- The emerging role of transcriptional regulation in the oocyte-to-zygote transition.PLoS genetics · 2020Article
- Gcn5 and Rpd3 have a limited role in the regulation of cell cycle transcripts during the G1 and S phases in Saccharomyces cerevisiae.Scientific reports · 2019Article
- A SIR-independent role for cohesin in subtelomeric silencing and organization.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Genetic and epigenetic determinants establish a continuum of Hsf1 occupancy and activity across the yeast genome.Molecular biology of the cell · 2018Article
- Chromatin accessibility dynamics acrosseLife · 2018Article
- The Nucleosome Remodeling and Deacetylation Complex Modulates Chromatin Structure at Sites of Active Transcription to Fine-Tune Gene Expression.Molecular cell · 2018Article
- Targeted Gene Repression Using Novel Bifunctional Molecules to Harness Endogenous Histone Deacetylation Activity.ACS synthetic biology · 2018Article
- Heat Shock Protein Genes Undergo Dynamic Alteration in Their Three-Dimensional Structure and Genome Organization in Response to Thermal Stress.Molecular and cellular biology · 2017Article
- Activation of MouseJournal of immunology (Baltimore, Md. : 1950) · 2017Article
- Use of conditioned media is critical for studies of regulation in response to rapid heat shock.Cell stress & chaperones · 2017Article
- Transcriptional Activation of Pericentromeric Satellite Repeats and Disruption of Centromeric Clustering upon Proteasome Inhibition.PloS one · 2016Article
- Absence of canonical marks of active chromatin in developmentally regulated genes.Nature genetics · 2015Article
- Tissue-specific epigenetics in gene neighborhoods: myogenic transcription factor genes.Human molecular genetics · 2015Article
- Genome distribution of replication-independent histone H1 variants shows H1.0 associated with nucleolar domains and H1X associated with RNA polymerase II-enriched regions.The Journal of biological chemistry · 2015Article
- Epigenetic Alterations in Alzheimer's Disease.Frontiers in behavioral neuroscience · 2015Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is widely accepted that transcriptional regulation of eukaryotic genes is intimately coupled to covalent modifications of the underlying chromatin template, and in certain cases the functional consequences of these modifications have been characterized. Here we present evidence that gene activation in the silent heterochromatin of the yeast Saccharomyces cerevisiae can occur in the context of little, if any, covalent histone modification. Using a SIR-regulated heat shock-inducible transgene, hsp82-2001, and a natural drug-inducible subtelomeric gene, YFR057w, as models we demonstrate that substantial transcriptional induction (>200-fold) can occur in the context of restricted histone loss and negligible levels of H3K4 trimethylation, H3K36 trimethylation and H3K79 dimethylation, modifications commonly linked to transcription initiation and elongation. Heterochromatic gene activation can also occur with minimal H3 and H4 lysine acetylation and without replacement of H2A with the transcription-linked variant H2A.Z. Importantly, absence of histone modification does not stem from reduced transcriptional output, since hsp82-ΔTATA, a euchromatic promoter mutant lacking a TATA box and with threefold lower induced transcription than heterochromatic hsp82-2001, is strongly hyperacetylated in response to heat shock. Consistent with negligible H3K79 dimethylation, dot1Δ cells lacking H3K79 methylase activity show unimpeded occupancy of RNA polymerase II within activated heterochromatic promoter and coding regions. Our results indicate that large increases in transcription can be observed in the virtual absence of histone modifications often thought necessary for gene activation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.