ArticlePloS one2014
Melanoma transition is frequently accompanied by a loss of cytoglobin expression in melanocytes: a novel expression site of cytoglobin.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- Cytoglobin attenuates melanoma malignancy but protects melanoma cells from ferroptosis.Molecular medicine reports · 2024Article
- The cytoglobin-dependent transcriptome in melanoma indicates a protective function associated with oxidative stress, inflammation and cancer-associated pathways.Scientific reports · 2024Article
- Effects of Nitro-Oxidative Stress on Biomolecules: Part 1-Non-Reactive Molecular Dynamics Simulations.Biomolecules · 2023Review
- Cytoglobin inhibits non-thermal plasma-induced apoptosis in melanoma cells through regulation of the NRF2-mediated antioxidant response.Redox biology · 2022Article
- Cytoglobin Silencing Promotes Melanoma Malignancy but Sensitizes for Ferroptosis and Pyroptosis Therapy Response.Antioxidants (Basel, Switzerland) · 2022Article
- Cytoglobin has potent superoxide dismutase function.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- A reliable set of reference genes to normalize oxygen-dependent cytoglobin gene expression levels in melanoma.Scientific reports · 2021Article
- Cytoglobin protects cancer cells from apoptosis by regulation of mitochondrial cardiolipin.Scientific reports · 2021Article
- Regulation of Nitric Oxide Metabolism and Vascular Tone by Cytoglobin.Antioxidants & redox signaling · 2020Review
- Emerging perspectives on cytoglobin, beyond NO dioxygenase and peroxidase.Redox biology · 2020Review
- Article
- Absence of cytoglobin promotes multiple organ abnormalities in aged mice.Scientific reports · 2016Article
- Discovery of cytoglobin and its roles in physiology and pathology of hepatic stellate cells.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2016Review
- Cytoglobin as a Marker of Hepatic Stellate Cell-derived Myofibroblasts.Frontiers in physiology · 2015Review
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
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Abstract
The tissue distribution and function of hemoglobin or myoglobin are well known; however, a newly found cytoglobin (CYGB), which also belongs to the globin family, remains to be characterized. To assess its expression in human malignancies, we sought to screen a number of cell lines originated from many tissues using northern blotting and real time PCR techniques. Unexpectedly, we found that several, but not all, melanoma cell lines expressed CYGB mRNA and protein at much higher levels than cells of other origins. Melanocytes, the primary origin of melanoma, also expressed CYGB at a high level. To verify these observations, immunostaining and immunoblotting using anti-CYGB antibody were also performed. Bisulfite-modified genomic sequencing revealed that several melanoma cell lines that abrogated CYGB expression were found to be epigenetically regulated by hypermethylation in the promoter region of CYGB gene. The RNA interference-mediated knockdown of the CYGB transcript in CYGB expression-positive melanoma cell lines resulted in increased proliferation in vitro and in vivo. Flow cytometric analysis using 2'-, 7'-dichlorofluorescein diacetate (DCFH-DA), an indicator of reactive oxygen species (ROS), revealed that the cellular ROS level may be involved in the proliferative effect of CYGB. Thus, CYGB appears to play a tumor suppressive role as a ROS regulator, and its epigenetic silencing, as observed in CYGB expression-negative melanoma cell lines, might function as an alternative pathway in the melanocyte-to-melanoma transition.
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