ArticleNature protocols2014
3D live fluorescence imaging of cellular dynamics using Bessel beam plane illumination microscopy.
Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 103 papers.
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Who cites it
103 citing papers in PubMed.
- Aberration correction and complementary beam subtraction in light-sheet fluorescence microscopy.Journal of biomedical optics · 2026Article
- Scanning Bessel beam microscopy with a protected and corrective objective for solvent-cleared large samples.iScience · 2026Article
- Tunable acoustic rotation for deep biophysical phenotyping of preclinical Alzheimer's disease.Materials today. Bio · 2026Article
- Article
- Single-Objective Lattice Light Sheet Microscopy with Microfluidics for Single-Molecule Super-Resolution Imaging of Mammalian Cells.ACS photonics · 2026Article
- Altair-LSFM A High-Resolution, Easy-to-Build Light-Sheet Microscope for Sub-Cellular Imaging.bioRxiv : the preprint server for biology · 2025Article
- Water-Immersed GaP Huygens' Meta-Optics for Visible Structured Light Generation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- A versatile and open source design for one and two photon light sheet microscopy.Scientific reports · 2025Article
- Efficient generation of Bessel-Gauss attosecond pulse trains via nonadiabatic phase-matched high-order harmonics.Light, science & applications · 2025Article
- Unsupervised spatiotemporal classification of deformation patterns of embryonic tissues matches their fate map.iScience · 2025Article
- A Single Sub-Millimetric Metasurface-Based Optical Element for Lattice Bessel Beam Excitation Enabling Brain Activity Recordings In Vivo.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Sidelobe suppressed Bessel beams for one-photon light-sheet microscopy.Biomedical optics express · 2024Article
- Frontiers in artificial intelligence-directed light-sheet microscopy for uncovering biological phenomena and multi-organ imaging.View (Beijing, China) · 2024Article
- Applications of Lightsheet Fluorescence Microscopy by High Numerical Aperture Detection Lens.The journal of physical chemistry. B · 2024Review
- Freestanding bilayer microscope for single-molecule imaging of membrane proteins.Science advances · 2024Article
- descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques.Nature communications · 2024Article
- Optical microscopic imaging, manipulation, and analysis methods for morphogenesis research.Microscopy (Oxford, England) · 2024Review
- Speckle-enabled in vivo demixing of neural activity in the mouse brain.Biomedical optics express · 2024Article
- Selective-plane-activation structured illumination microscopy.Nature methods · 2024Article
- Article
43 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors.
Funding
Abstract
3D live imaging is important for a better understanding of biological processes, but it is challenging with current techniques such as spinning-disk confocal microscopy. Bessel beam plane illumination microscopy allows high-speed 3D live fluorescence imaging of living cellular and multicellular specimens with nearly isotropic spatial resolution, low photobleaching and low photodamage. Unlike conventional fluorescence imaging techniques that usually have a unique operation mode, Bessel plane illumination has several modes that offer different performance with different imaging metrics. To achieve optimal results from this technique, the appropriate operation mode needs to be selected and the experimental setting must be optimized for the specific application and associated sample properties. Here we explain the fundamental working principles of this technique, discuss the pros and cons of each operational mode and show through examples how to optimize experimental parameters. We also describe the procedures needed to construct, align and operate a Bessel beam plane illumination microscope by using our previously reported system as an example, and we list the necessary equipment to build such a microscope. Assuming all components are readily available, it would take a person skilled in optical instrumentation ∼1 month to assemble and operate a microscope according to this protocol.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.