ArticleNature protocols2014
Quantifying the forces guiding microbial cell adhesion using single-cell force spectroscopy.
Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers, 1 of them a synthesis that pooled it.
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Who cites it
65 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A detailed guideline for the fabrication of single bacterial probes used for atomic force spectroscopy.The European physical journal. E, Soft matter · 2015Guideline
- A biofilm-derived peptide as an underwater adhesive.bioRxiv : the preprint server for biology · 2026Article
- Physical Changes of Biomacromolecules upon Covalent Surface Immobilization.Langmuir : the ACS journal of surfaces and colloids · 2026Article
- Meta-surface plasmon resonance microscopy for sensitive, high-throughput, and long-term label-free analysis of cell adhesion dynamics.Journal of nanobiotechnology · 2025Article
- Micro- and nano-scale adhesion of oral bacteria to biomaterials using atomic force microscopy: A systematic review.The Japanese dental science review · 2025Review
- Femtosecond laser irradiation has an anti-virulence effect by reducing the adhesion and invasion of MRSA bacteria to human cells: an in vitro study.Lasers in medical science · 2025Article
- Single-Cell Force Spectroscopy Uncovers Root Zone- and Bacteria-Specific Interactions.Angewandte Chemie (International ed. in English) · 2025Article
- Dental plaque-inspired peptide engineered to control plaque accumulation.Materials today. Bio · 2025Article
- Article
- Probing living cell dynamics and molecular interactions using atomic force microscopy.Biophysical reviews · 2024Review
- Evaluation of Conductive Porous Biobased Composites with Tunable Mechanical Properties for Potential Biological Applications.ACS omega · 2024Article
- Characterization of a unique attachment organelle: Single-cell force spectroscopy ofNanoscale · 2024Article
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- Quantum-enhanced diamond molecular tension microscopy for quantifying cellular forces.Science advances · 2024Article
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- The Determination, Monitoring, Molecular Mechanisms and Formation of Biofilm in E. coli.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Review
- Extending applications of AFM to fluidic AFM in single living cell studies.Journal of cellular physiology · 2022Review
- Unraveling the binding microprocess of individualJournal of oral microbiology · 2022Article
- Quantifying molecular- to cellular-level forces in living cells.Journal of physics D: Applied physics · 2021Article
- Self-adhesive lubricated coating for enhanced bacterial resistance.Bioactive materials · 2021Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During the past decades, several methods (e.g., electron microscopy, flow chamber experiments, surface chemical analysis, surface charge and surface hydrophobicity measurements) have been developed to investigate the mechanisms controlling the adhesion of microbial cells to other cells and to various other substrates. However, none of the traditional approaches are capable of looking at adhesion forces at the single-cell level. In recent years, atomic force microscopy (AFM) has been instrumental in measuring the forces driving microbial adhesion on a single-cell basis. The method, known as single-cell force spectroscopy (SCFS), consists of immobilizing a single living cell on an AFM cantilever and measuring the interaction forces between the cellular probe and a solid substrate or another cell. Here we present SCFS protocols that we have developed for quantifying the cell adhesion forces of medically important microbes. Although we focus mainly on the probiotic bacterium Lactobacillus plantarum, we also show that our procedures are applicable to pathogens, such as the bacterium Staphylococcus epidermidis and the yeast Candida albicans. For well-trained microscopists, the entire protocol can be mastered in 1 week.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.