ArticleScientific reports2014
Enzyme-free passage of human pluripotent stem cells by controlling divalent cations.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 43 citations in OpenAlex.
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- Selection of Mechanical Fragmentation Methods Based on Enzyme-Free Preparation of Decellularized Adipose-Derived Matrix.Bioengineering (Basel, Switzerland) · 2023Article
- DUSP6 is a memory retention feedback regulator of ERK signaling for cellular resilience of human pluripotent stem cells in response to dissociation.Scientific reports · 2023Article
- Identification of a familial cleidocranial dysplasia with a novel RUNX2 mutation and establishment of patient-derived induced pluripotent stem cells.Odontology · 2022Article
- Development and evaluation of a novel xeno-free culture medium for human-induced pluripotent stem cells.Stem cell research & therapy · 2022Article
- Detachment of cell sheets from clinically ubiquitous cell culture vessels by ultrasonic vibration.Scientific reports · 2020Article
- Induction of integration-free human-induced pluripotent stem cells under serum- and feeder-free conditions.In vitro cellular & developmental biology. Animal · 2020Article
- Enzyme-free release of adhered cells from standard culture dishes using intermittent ultrasonic traveling waves.Communications biology · 2019Article
- Random migration of induced pluripotent stem cell-derived human gastrulation-stage mesendoderm.PloS one · 2018Article
- Thalidomide induces apoptosis in undifferentiated human induced pluripotent stem cells.In vitro cellular & developmental biology. Animal · 2017Article
- Generation of iPSC-derived Human Brain Organoids to Model Early Neurodevelopmental Disorders.Journal of visualized experiments : JoVE · 2017Article
- Imaging-cytometry revealed spatial heterogeneities of marker expression in undifferentiated human pluripotent stem cells.In vitro cellular & developmental biology. Animal · 2017Article
- Effect of enzymatic and mechanical methods of dissociation on neural progenitor cells derived from induced pluripotent stem cells.Advances in medical sciences · 2016Article
- Generation of cleidocranial dysplasia-specific human induced pluripotent stem cells in completely serum-, feeder-, and integration-free culture.In vitro cellular & developmental biology. Animal · 2016Article
- Development of a Monitoring Method for Nonlabeled Human Pluripotent Stem Cell Growth by Time-Lapse Image Analysis.Stem cells translational medicine · 2015Article
- Manufacturing of dental pulp cell-based products from human third molars: current strategies and future investigations.Frontiers in physiology · 2015Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enzymes used for passaging human pluripotent stem cells (hPSCs) digest cell surface proteins, resulting in cell damage. Moreover, cell dissociation using divalent cation-free solutions causes apoptosis. Here we report that Mg(2+) and Ca(2+) control cell-fibronectin and cell-cell binding of hPSCs, respectively, under feeder- and serum-free culture conditions without enzyme. The hPSCs were detached from fibronectin-, vitronectin- or laminin-coated dishes in low concentrations of Mg(2+) and remained as large colonies in high concentrations of Ca(2+). Using enzyme-free solutions containing Ca(2+) without Mg(2+), we successfully passaged hPSCs as large cell clumps that showed less damage than cells passaged using a divalent cation-free solution or dispase. Under the same conditions, the undifferentiated and early-differentiated cells could also be harvested as a cell sheet without being split off. Our enzyme-free passage of hPSCs under a serum- and feeder-free culture condition reduces cell damage and facilitates easier and safer cultures of hPSCs.
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