ArticleViruses2014
Superresolution imaging of human cytomegalovirus vMIA localization in sub-mitochondrial compartments.
Article in Viruses, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 33 citations in OpenAlex.
- Mitochondrial changes associated with viral infectious diseases in the paediatric population.Reviews in medical virology · 2021Pooled it
- Localization of the Remnant of a Cell Wall Sorting Signal and Its Interaction with a Sensor Kinase.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Advanced imaging techniques for tracking drug dynamics at the subcellular level.Advanced drug delivery reviews · 2023Review
- The roles of autophagy and mitophagy in corneal pathology: current knowledge and future perspectives.Frontiers in medicine · 2023Review
- Restructured membrane contacts rewire organelles for human cytomegalovirus infection.Nature communications · 2022Article
- Organelle Interaction and Drug Discovery: Towards Correlative Nanoscopy and Molecular Dynamics Simulation.Frontiers in pharmacology · 2022Review
- Herpesvirus Regulation of Selective Autophagy.Viruses · 2021Review
- Mitochondrial Enzymes of the Urea Cycle Cluster at the Inner Mitochondrial Membrane.Frontiers in physiology · 2020Article
- Human Cytomegalovirus pUL37x1 Is Important for Remodeling of Host Lipid Metabolism.Journal of virology · 2019Article
- Improved spatial resolution by induced live cell and organelle swelling in hypotonic solutions.Scientific reports · 2019Article
- Mitochondrial Morphofunction in Mammalian Cells.Antioxidants & redox signaling · 2019Review
- An intramembrane sensory circuit monitors sortase A-mediated processing of streptococcal adhesins.Science signaling · 2019Article
- Endoplasmic Reticulum-Mitochondrial Contactology: Structure and Signaling Functions.Trends in cell biology · 2018Review
- Superresolution Imaging Identifies That Conventional Trafficking Pathways Are Not Essential for Endoplasmic Reticulum to Outer Mitochondrial Membrane Protein Transport.Scientific reports · 2017Article
- Drp1-dependent mitochondrial fission via MiD49/51 is essential for apoptotic cristae remodeling.The Journal of cell biology · 2016Article
- Isolation of Endoplasmic Reticulum, Mitochondria, and Mitochondria-Associated Membrane and Detergent Resistant Membrane Fractions from Transfected Cells and from Human Cytomegalovirus-Infected Primary Fibroblasts.Current protocols in cell biology · 2015Article
- Superresolution imaging of viral protein trafficking.Medical microbiology and immunology · 2015Review
- Oncogenic and oncosuppressive signal transduction at mitochondria-associated endoplasmic reticulum membranes.Molecular & cellular oncologyReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
The human cytomegalovirus (HCMV) viral mitochondria-localized inhibitor of apoptosis (vMIA) protein, traffics to mitochondria-associated membranes (MAM), where the endoplasmic reticulum (ER) contacts the outer mitochondrial membrane (OMM). vMIA association with the MAM has not been visualized by imaging. Here, we have visualized this by using a combination of confocal and superresolution imaging. Deconvolution of confocal microscopy images shows vMIA localizes away from mitochondrial matrix at the Mitochondria-ER interface. By gated stimulated emission depletion (GSTED) imaging, we show that along this interface vMIA is distributed in clusters. Through multicolor, multifocal structured illumination microscopy (MSIM), we find vMIA clusters localize away from MitoTracker Red, indicating its OMM localization. GSTED and MSIM imaging show vMIA exists in clusters of ~100-150 nm, which is consistent with the cluster size determined by Photoactivated Localization Microscopy (PALM). With these diverse superresolution approaches, we have imaged the clustered distribution of vMIA at the OMM adjacent to the ER. Our findings directly compare the relative advantages of each of these superresolution imaging modalities for imaging components of the MAM and sub-mitochondrial compartments. These studies establish the ability of superresolution imaging to provide valuable insight into viral protein location, particularly in the sub-mitochondrial compartments, and into their clustered organization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.