ArticleActa neuropathologica1989
Differential expression of glial fibrillary acidic protein in human glioma cell lines.
Article in Acta neuropathologica, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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7 citing papers in PubMed, 14 citations in OpenAlex.
- A novel human astrocyte cell line (A735) with astrocyte-specific neurotransmitter function.In vitro cellular & developmental biology. Animal · 1999Article
- Suppression of TGF-beta1 in human gliomas by retroviral gene transfection enhances susceptibility to LAK cells.Journal of neuro-oncology · 1999Article
- Cytokine gene expression on glioma cell lines and specimens.Journal of neuro-oncology · 1994Article
- Differential expression of myc, max and RB1 genes in human gliomas and glioma cell lines.British journal of cancer · 1994Article
- A novel human glioma cell line from pituitaries.In vitro cellular & developmental biology : journal of the Tissue Culture Association · 1993Article
- Human glial fibrillary acidic protein (GFAP): molecular cloning of the complete cDNA sequence and chromosomal localization (chromosome 17) of the GFAP gene.Acta neuropathologica · 1992Article
- Changes in glial fibrillary acidic protein and karyotype during culturing of two cell lines established from human glioblastoma multiforme.Cell and tissue research · 1991Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have obtained a cDNA fragment to human glial fibrillary acidic protein (GFAP) by immunoscreening a lambda gt11 human brain cDNA library with antibody to bovine GFAP. The highly homologous nucleotide sequence of this clone with that of the mouse GFAP enabled the identification of this cDNA as one encoding GFAP. As this cDNA hybridized with a single major RNA species in Northern blots of RNA from human and mouse brain tissues and gave one or two bands in Southern blots of human genomic DNA, it was considered to be specific for GFAP. Using this cDNA as a probe we investigated the levels of GFAP expression in ten human glioma cell lines. A 3.5-kb GFAP mRNA was detected in five of the ten glioma cell lines, one of which was U-251 MG cell line and the other four were clones derived from the same tumor (CL1, 2, 3, and 4). There was a difference in the amount of GFAP mRNA among U-251 MG and the four clonal cell lines. Quantitative evaluation of this difference by RNA dot blot analysis revealed that the amount of GFAP mRNA expressed in CL3 was about 1/5 and in CL4 about 1/10 the amount expressed in U-251 MG, CL1, and CL2. Semiquantitative Western blot analysis showed that GFAP levels corresponded to the GFAP mRNA levels in these cell lines. By Southern blot analysis of genomic DNA the GFAP gene was similarly detected in all of these cell lines regardless of the level of GFAP expression.(ABSTRACT TRUNCATED AT 250 WORDS)
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