ArticleJournal of clinical microbiology2014
Long-term stability at -20 °C of Aspergillus galactomannan in serum and bronchoalveolar lavage specimens.
Article in Journal of clinical microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- Prospective Evaluation of Galactomannan and (1→3) β-d-Glucan Assays as Diagnostic Tools for Invasive Fungal Disease in Children, Adolescents, and Young Adults With Acute Myeloid Leukemia Receiving Fungal Prophylaxis.Journal of the Pediatric Infectious Diseases Society · 2021Trial
- Assessment of the performances of blood tests for the antemortem diagnosis of aspergillosis in wild or captive aquatic birds.Veterinary research · 2026Article
- Retrospective Multicenter Evaluation of the VirClia Galactomannan Antigen Assay for the Diagnosis of Pulmonary Aspergillosis with Bronchoalveolar Lavage Fluid Samples from Patients with Hematological Disease.Journal of clinical microbiology · 2023Article
- Aspergillus Lateral Flow Assay with Digital Reader for the Diagnosis of COVID-19-Associated Pulmonary Aspergillosis (CAPA): a Multicenter Study.Journal of clinical microbiology · 2022Article
- Impact of Changes of the 2020 Consensus Definitions of Invasive Aspergillosis on Clinical Trial Design: Unintended Consequences for Prevention Trials?Open forum infectious diseases · 2021Article
- PCR-Based Approach Targeting Mucorales-Specific Gene Family for Diagnosis of Mucormycosis.Journal of clinical microbiology · 2018Article
- Improving survival of acute-on-chronic liver failure patients complicated with invasive pulmonary aspergillosis.Scientific reports · 2018Article
- Improved Standardization of the Bio-Rad Platelia Aspergillus Galactomannan Antigen Sandwich Enzyme Immunoassay Using the DS2 (Dynex) Enzyme-Linked Immunosorbent Assay (ELISA) Processing System.Journal of clinical microbiology · 2015Article
- Importance of operational factors in the reproducibility of Aspergillus galactomannan enzyme immune assay.PloS one · 2015Article
- Instability of Aspergillus galactomannan in stored clinical samples.Journal of clinical microbiology · 2014Article
- Reply to "Insufficient demonstration of long-term stability of Aspergillus galactomannan".Journal of clinical microbiology · 2014Article
- Insufficient demonstration of long-term stability of Aspergillus galactomannan.Journal of clinical microbiology · 2014Article
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14 authors at 9 institutions in 2 countries.
Funding
Abstract
Research to develop and validate novel methods for diagnosis of aspergillosis based on detection of galactomannan requires the use of clinical specimens that have been stored frozen. Data indicating that galactomannan remains stable when frozen are scant. The objective of this study was to determine the stability of galactomannan in clinical specimens stored at -20 °C that were positive in the Platelia Aspergillus enzyme immunoassay when initially tested. Prospective real-time testing of serum and bronchoalveolar lavage (BAL) fluid pools from positive and negative patient specimens showed no decline in galactomannan index (GMI) over 11 months at -20 °C and no development of positive reactions in the negative-control pool. Retrospective testing of positive specimens that had been stored at -20 °C for 5 years showed that 28 of 30 serum (n = 15) or BAL (n = 15) specimens remained positive. These findings support the use of frozen serum or BAL specimens stored for at least 5 years in evaluation of diagnostic tests based on detection of galactomannan.
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