ArticleDevelopment (Cambridge, England)2014
miR-8 controls synapse structure by repression of the actin regulator enabled.
Article in Development (Cambridge, England), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 43 citations in OpenAlex.
- The miR-8/miR-200 family: from functional diversification to translational applications.Cellular & molecular biology letters · 2026Review
- MicroRNAs in the biology and hallmarks of neurodegenerative diseases.Trends in molecular medicine · 2025Review
- Insights into the microRNA landscape of Rhodnius prolixus, a vector of Chagas disease.Scientific reports · 2023Article
- Argonaute 2 is lost from neuromuscular junctions affected with amyotrophic lateral sclerosis in SOD1Scientific reports · 2022Article
- Fe65: A Scaffolding Protein of Actin Regulators.Cells · 2021Review
- Drosophila primary microRNA-8 encodes a microRNA-encoded peptide acting in parallel of miR-8.Genome biology · 2021Article
- Insulin signaling mediates neurodegeneration in glioma.Life science alliance · 2021Article
- Synapse development and maturation at the drosophila neuromuscular junction.Neural development · 2020Review
- Drosophila enabled promotes synapse morphogenesis and regulates active zone form and function.Neural development · 2020Article
- The conserved microRNA miR-34 regulates synaptogenesis via coordination of distinct mechanisms in presynaptic and postsynaptic cells.Nature communications · 2020Article
- MicroRNAs Regulate Multiple Aspects of Locomotor Behavior inG3 (Bethesda, Md.) · 2020Article
- Neurexin and Neuroligins Maintain the Balance of Ghost and Satellite Boutons at theFrontiers in neuroanatomy · 2020Article
- MicroRNAs in Drosophila Cancer Models.Advances in experimental medicine and biology · 2019Review
- miRNA in spinal muscular atrophy pathogenesis and therapy.Journal of cellular and molecular medicine · 2018Review
- MicroRNA Tissue Atlas of the Malaria MosquitoG3 (Bethesda, Md.) · 2018Article
- MicroRNA function in Drosophila melanogaster.Seminars in cell & developmental biology · 2017Review
- MotomiRs: miRNAs in Motor Neuron Function and Disease.Frontiers in molecular neuroscience · 2017Review
- The Strip-Hippo Pathway Regulates Synaptic Terminal Formation by Modulating Actin Organization at the Drosophila Neuromuscular Synapses.Cell reports · 2016Article
- Cell-type-specific miR-431 dysregulation in a motor neuron model of spinal muscular atrophy.Human molecular genetics · 2016Article
- miR-8 modulates cytoskeletal regulators to influence cell survival and epithelial organization in Drosophila wings.Developmental biology · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
MicroRNAs (miRNAs) are post-transcriptional regulators of gene expression that play important roles in nervous system development and physiology. However, our understanding of the strategies by which miRNAs control synapse development is limited. We find that the highly conserved miRNA miR-8 regulates the morphology of presynaptic arbors at the Drosophila neuromuscular junction (NMJ) through a postsynaptic mechanism. Developmental analysis shows that miR-8 is required for presynaptic expansion that occurs in response to larval growth of the postsynaptic muscle targets. With an in vivo sensor, we confirm our hypothesis that the founding member of the conserved Ena/VASP (Enabled/Vasodilator Activated Protein) family is regulated by miR-8 through a conserved site in the Ena 3' untranslated region (UTR). Synaptic marker analysis and localization studies suggest that Ena functions within the subsynaptic reticulum (SSR) surrounding presynaptic terminals. Transgenic lines that express forms of a conserved mammalian Ena ortholog further suggest that this localization and function of postsynaptic Ena/VASP family protein is dependent on conserved C-terminal domains known to mediate actin binding and assembly while antagonizing actin-capping proteins. Ultrastructural analysis demonstrates that miR-8 is required for SSR morphogenesis. As predicted by our model, we find that Ena is both sufficient and necessary to account for miR-8-mediated regulation of SSR architecture, consistent with its localization in this compartment. Finally, electrophysiological analysis shows that miR-8 is important for spontaneous neurotransmitter release frequency and quantal content. However, unlike the structural phenotypes, increased expression of Ena fails to mimic the functional defects observed in miR-8-null animals. Together, these findings suggest that miR-8 limits the expansion of presynaptic terminals during larval synapse development through regulation of postsynaptic actin assembly that is independent of changes in synapse physiology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.