ArticleMethods in molecular biology (Clifton, N.J.)2014
Light-inducible gene regulation with engineered zinc finger proteins.
Article in Methods in molecular biology (Clifton, N.J.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- Programmable genome engineering and gene modifications for plant biodesign.Plant communications · 2025Review
- A Dual-Purpose Real-Time Indicator and Transcriptional Integrator for Calcium Detection in Living Cells.ACS synthetic biology · 2022Article
- Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells.Journal of visualized experiments : JoVE · 2021Article
- A method to specifically activate the Klotho promoter by using zinc finger proteins constructed from modular building blocks and from naturally engineered Egr1 transcription factor backbone.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Dual Activation of cAMP Production Through Photostimulation or Chemical Stimulation.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Genetic and epigenetic editing in nervous system .Dialogues in clinical neuroscience · 2019Review
- Article
- Bringing Light to Transcription: The Optogenetics Repertoire.Frontiers in genetics · 2018Review
- An Engineered Optogenetic Switch for Spatiotemporal Control of Gene Expression, Cell Differentiation, and Tissue Morphogenesis.ACS synthetic biology · 2017Article
- Article
- A light-inducible CRISPR-Cas9 system for control of endogenous gene activation.Nature chemical biology · 2015Article
- Natural photoreceptors as a source of fluorescent proteins, biosensors, and optogenetic tools.Annual review of biochemistry · 2015Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The coupling of light-inducible protein-protein interactions with gene regulation systems has enabled the control of gene expression with light. In particular, heterodimer protein pairs from plants can be used to engineer a gene regulation system in mammalian cells that is reversible, repeatable, tunable, controllable in a spatiotemporal manner, and targetable to any DNA sequence. This system, Light-Inducible Transcription using Engineered Zinc finger proteins (LITEZ), is based on the blue light-induced interaction of GIGANTEA and the LOV domain of FKF1 that drives the localization of a transcriptional activator to the DNA-binding site of a highly customizable engineered zinc finger protein. This chapter provides methods for modifying LITEZ to target new DNA sequences, engineering a programmable LED array to illuminate cell cultures, and using the modified LITEZ system to achieve spatiotemporal control of transgene expression in mammalian cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.