ArticleOpen biology2014
An endogenous green fluorescent protein-photoprotein pair in Clytia hemisphaerica eggs shows co-targeting to mitochondria and efficient bioluminescence energy transfer.
Article in Open biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 41 citations in OpenAlex.
- Fast kinetics of photoprotein emitting species.Scientific reports · 2025Article
- Design of Ctenophore CaLife (Basel, Switzerland) · 2024Article
- The Role of Tyr-His-Trp Triad and Water Molecule Near the N1-Atom of 2-Hydroperoxycoelenterazine in Bioluminescence of Hydromedusan Photoproteins: Structural and Mutagenesis Study.International journal of molecular sciences · 2023Article
- A chromosome-scale epigenetic map of theGenome research · 2023Article
- siRNA-mediated gene knockdown via electroporation in hydrozoan jellyfish embryos.Scientific reports · 2022Article
- Whole-animal multiplexed single-cell RNA-seq reveals transcriptional shifts acrossScience advances · 2021Article
- Article
- Unusual shift in the visible absorption spectrum of an active ctenophore photoprotein elucidated by time-dependent density functional theory.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2021Article
- Sea as a color palette: the ecology and evolution of fluorescence.Zoological letters · 2020Review
- The genome of the jellyfish Clytia hemisphaerica and the evolution of the cnidarian life-cycle.Nature ecology & evolution · 2019Article
- High doses of CRISPR/Cas9 ribonucleoprotein efficiently induce gene knockout with low mosaicism in the hydrozoan Clytia hemisphaerica through microhomology-mediated deletion.Scientific reports · 2018Article
- Frame-Insensitive Expression Cloning of Fluorescent Protein from Scolionema suvaense.International journal of molecular sciences · 2018Article
- Non-excitable fluorescent protein orthologs found in ctenophores.BMC evolutionary biology · 2016Article
- Oocytes express an endogenous red fluorescent protein in a stony coral, Euphyllia ancora: a potential involvement in coral oogenesis.Scientific reports · 2016Article
- Green Fluorescence of Cytaeis Hydroids Living in Association with Nassarius Gastropods in the Red Sea.PloS one · 2016Article
- Fluorescent proteins function as a prey attractant: experimental evidence from the hydromedusa Olindias formosus and other marine organisms.Biology open · 2015Article
- Differential responses to Wnt and PCP disruption predict expression and developmental function of conserved and novel genes in a cnidarian.PLoS genetics · 2014Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Green fluorescent proteins (GFPs) and calcium-activated photoproteins of the aequorin/clytin family, now widely used as research tools, were originally isolated from the hydrozoan jellyfish Aequora victoria. It is known that bioluminescence resonance energy transfer (BRET) is possible between these proteins to generate flashes of green light, but the native function and significance of this phenomenon is unclear. Using the hydrozoan Clytia hemisphaerica, we characterized differential expression of three clytin and four GFP genes in distinct tissues at larva, medusa and polyp stages, corresponding to the major in vivo sites of bioluminescence (medusa tentacles and eggs) and fluorescence (these sites plus medusa manubrium, gonad and larval ectoderms). Potential physiological functions at these sites include UV protection of stem cells for fluorescence alone, and prey attraction and camouflaging counter-illumination for bioluminescence. Remarkably, the clytin2 and GFP2 proteins, co-expressed in eggs, show particularly efficient BRET and co-localize to mitochondria, owing to parallel acquisition by the two genes of mitochondrial targeting sequences during hydrozoan evolution. Overall, our results indicate that endogenous GFPs and photoproteins can play diverse roles even within one species and provide a striking and novel example of protein coevolution, which could have facilitated efficient or brighter BRET flashes through mitochondrial compartmentalization.
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