ArticleReproductive sciences (Thousand Oaks, Calif.)2014
Molecular cytogenetics of human single pronucleated zygotes.
Article in Reproductive sciences (Thousand Oaks, Calif.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- A large retrospective study on 1PN embryo transfer supports the need for updated harmonized best practice guidelines.Human reproduction (Oxford, England) · 2026Article
- Could blastocysts derived from abnormal fertilized zygotes be used? A systematic review and meta-analysis.European journal of obstetrics & gynecology and reproductive biology: X · 2025Review
- The Identification of Molecular Ploidy Status of Abnormal Pronuclear Zygotes Reveals a Significant Number of Euploid Blastocysts Available for Conception.Biomedicines · 2024Article
- Incidence of haploidy and triploidy in trophectoderm biopsies of blastocysts derived from normally and abnormally fertilized oocytes.Journal of assisted reproduction and genetics · 2024Article
- Birth of Thirty-Two Healthy Babies Following Transfer of Fresh and Frozen-Thawed Embryos Derived from Monopronuclear Zygotes: A Retrospective Study.Medicina (Kaunas, Lithuania) · 2024Article
- Prediction of live birth in vitrified-warmed 1PN-derived blastocyst transfer: Overall quality grade, ICM, TE, and expansion degree.Frontiers in physiology · 2022Article
- Comparison of the rates for reaching the blastocyst stage between normal and abnormal pronucleus embryos monitored by a time-lapse system in IVF patientsJournal of the Turkish German Gynecological Association · 2021Article
- HIRA contributes to zygote formation in mice and is implicated in human 1PN zygote phenotype.Reproduction (Cambridge, England) · 2021Article
- Chromosomal analysis of blastocyst derived from monopronucleated ICSI zygotes: approach by double trophectoderm biopsy.JBRA assisted reproduction · 2017Article
- Could monopronucleated ICSI zygotes be considered for transfer? Analysis through time-lapse monitoring and PGS.Journal of assisted reproduction and genetics · 2017Article
- Epigenetic reprogramming of the zygote in mice and men: on your marks, get set, go!Reproduction (Cambridge, England) · 2016Review
- Early Fertilization Abnormalities in the Human: An Exhausted Area of Research?Journal of reproduction & infertilityArticle
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of the present study was to use fluorescence in situ hybridization to analyze the chromosome status of zygotes with a single pronucleus from in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) treatment cycles. In addition, we performed immunocytochemical detection of nuclear lamins and histone H3 trimethylated at lysine-9, Me(3)H3K9. Zygotes were processed 24 hours after insemination or injection to assure the absence of asynchrony. In opposition to previous results, we observed 2 pronuclei in 16 of 18 IVF zygotes and 40 of 64 ICSI zygotes, suggesting premature pronuclear breakdown. In IVF and ICSI zygotes, the rate of normal diploidy was only 6 of 16 and 27 of 56, respectively, suggesting that monopronucleated zygotes should not be used in assisted reproductive treatments. The possible mechanisms are discussed and compared to previous studies of monopronucleated zygotes.
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