ArticleBiofabrication2014
3D printing facilitated scaffold-free tissue unit fabrication.
Article in Biofabrication, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
45 citing papers in PubMed, 149 citations in OpenAlex.
- Multifunctional Agarose-Based Biomaterials: From Tissue Engineering and Immunomodulation to Advanced Diagnostics and Translational Applications.Gels (Basel, Switzerland) · 2026Review
- Recent Advances in Hydrogel-Based 3D Bioprinting and Its Potential Application in the Treatment of Congenital Heart Disease.Biomolecules · 2024Review
- Biofabrication's Contribution to the Evolution of Cultured Meat.Advanced healthcare materials · 2024Review
- Synergistic coupling between 3D bioprinting and vascularization strategies.Biofabrication · 2023Review
- A pH/enzyme dual responsive PMB spatiotemporal release hydrogel promoting chronic wound repair.Journal of nanobiotechnology · 2023Article
- 3D Bioprinting for Vascularization.Bioengineering (Basel, Switzerland) · 2023Review
- Bioprinting Methods for Fabricating In Vitro Tubular Blood Vessel Models.Cyborg and bionic systems (Washington, D.C.) · 2023Review
- Biomaterial application strategies to enhance stem cell-based therapy for ischemic stroke.World journal of stem cells · 2022Article
- Application of three-dimensional printing technology in renal diseases.Frontiers in medicine · 2022Review
- Theoretical and Experimental Research on Multi-Layer Vessel-like Structure Printing Based on 3D Bio-Printing Technology.Micromachines · 2021Article
- 3D-printed silica with nanoscale resolution.Nature materials · 2021Article
- In Vitro Strategies to Vascularize 3D Physiologically Relevant Models.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- Integrated printed BDNF/collagen/chitosan scaffolds with low temperature extrusion 3D printer accelerated neural regeneration after spinal cord injury.Regenerative biomaterials · 2021Article
- Comparison of Osteogenic Potentials of Dental Pulp and Bone Marrow Mesenchymal Stem Cells Using the New Cell Transplantation Platform, CellSaic, in a Rat Congenital Cleft-Jaw Model.International journal of molecular sciences · 2021Article
- Prevascularized Micro-/Nano-Sized Spheroid/Bead Aggregates for Vascular Tissue Engineering.Nano-micro letters · 2021Article
- 3D cell aggregate printing technology and its applications.Essays in biochemistry · 2021Review
- Advances in removing mass transport limitations for more physiologically relevantBiophysics reviews · 2021Review
- Recent approaches in clinical applications of 3D printing in neonates and pediatrics.European journal of pediatrics · 2021Review
- Biomaterials for Bioprinting Microvasculature.Chemical reviews · 2020Review
- Bioprinting: From Tissue and Organ Development toChemical reviews · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
Tissue spheroids hold great potential in tissue engineering as building blocks to assemble into functional tissues. To date, agarose molds have been extensively used to facilitate fusion process of tissue spheroids. As a molding material, agarose typically requires low temperature plates for gelation and/or heated dispenser units. Here, we proposed and developed an alginate-based, direct 3D mold-printing technology: 3D printing microdroplets of alginate solution into biocompatible, bio-inert alginate hydrogel molds for the fabrication of scaffold-free tissue engineering constructs. Specifically, we developed a 3D printing technology to deposit microdroplets of alginate solution on calcium containing substrates in a layer-by-layer fashion to prepare ring-shaped 3D hydrogel molds. Tissue spheroids composed of 50% endothelial cells and 50% smooth muscle cells were robotically placed into the 3D printed alginate molds using a 3D printer, and were found to rapidly fuse into toroid-shaped tissue units. Histological and immunofluorescence analysis indicated that the cells secreted collagen type I playing a critical role in promoting cell-cell adhesion, tissue formation and maturation.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.