ArticleBiotechnology for biofuels2014
Production of free monounsaturated fatty acids by metabolically engineered Escherichia coli.
Article in Biotechnology for biofuels, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 106 citations in OpenAlex.
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- Abiotic factors improving fatty acid profiling of freshwater indigenous microalgae isolated from Kumaun region of Uttarakhand, India.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Longitudinal Single-Cell Imaging of Engineered Strains with Stimulated Raman Scattering to Characterize Heterogeneity in Fatty Acid Production.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Lipid production and cellular changes in Fremyella diplosiphon exposed to nanoscale zerovalent iron nanoparticles and ampicillin.Microbial cell factories · 2023Article
- Adaptation of Glycyrrhiza glabra L. to water deficiency based on carbohydrate and fatty acid quantity and quality.Scientific reports · 2023Article
- Fatty Acid Production by Enhanced Malonyl-CoA Supply in Escherichia coli.Current microbiology · 2022Article
- Lipid turnover and SQUAMOSA promoter-binding proteins mediate variation in fatty acid desaturation under early nitrogen deprivation revealed by lipidomic and transcriptomic analyses inFrontiers in plant science · 2022Article
- Abiotic and Biotic Damage of Microalgae Generate Different Volatile Organic Compounds (VOCs) for Early Diagnosis of Algal Cultures for Biofuel Production.Metabolites · 2021Article
- The use of flow cytometry to assessBiotechnology reports (Amsterdam, Netherlands) · 2021Article
- Improvement of Free Fatty Acid Secretory Productivity inFrontiers in microbiology · 2021Article
- Untargeted Lipidomics Analysis of the CyanobacteriumInternational journal of molecular sciences · 2020Article
- Revealing the Potential of Lipid and β-Glucans Coproduction inMicroorganisms · 2020Article
- Engineering Escherichia coli FAB system using synthetic plant genes for the production of long chain fatty acids.Microbial cell factories · 2019Article
- Comparison of Lipid and Palmitoleic Acid Induction ofInternational journal of molecular sciences · 2019Article
- Coupled biosynthesis and esterification of 1,2,4-butanetriol to simplify its separation from fermentation broth.Engineering in life sciences · 2019Article
- Production of long-chain free fatty acids from metabolically engineered Rhodobacter sphaeroides heterologously producing periplasmic phospholipase A2 in dodecane-overlaid two-phase culture.Microbial cell factories · 2019Article
- Hybrid liquid biphasic system for cell disruption and simultaneous lipid extraction from microalgaeBiotechnology for biofuels · 2019Article
- Structural determinant of functionality in acyl lipid desaturases.Journal of lipid research · 2018Article
- Membrane-Located Expression of Thioesterase FromFrontiers in microbiology · 2018Article
- Efficient production of free fatty acids from ionic liquid-based acid- or enzyme-catalyzed bamboo hydrolysate.Journal of industrial microbiology & biotechnology · 2017Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMonounsaturated fatty acids (MUFAs) are the best components for biodiesel when considering the low temperature fluidity and oxidative stability. However, biodiesel derived from vegetable oils or microbial lipids always consists of significant amounts of polyunsaturated and saturated fatty acids (SFAs) alkyl esters, which hampers its practical applications. Therefore, the fatty acid composition should be modified to increase MUFA contents as well as enhancing oil and lipid production.
resultsThe model microorganism Escherichia coli was engineered to produce free MUFAs. The fatty acyl-ACP thioesterase (AtFatA) and fatty acid desaturase (SSI2) from Arabidopsis thaliana were heterologously expressed in E. coli BL21 star(DE3) to specifically release free unsaturated fatty acids (UFAs) and convert SFAs to UFAs. In addition, the endogenous fadD gene (encoding acyl-CoA synthetase) was disrupted to block fatty acid catabolism while the native acetyl-CoA carboxylase (ACCase) was overexpressed to increase the malonyl coenzyme A (malonyl-CoA) pool and boost fatty acid biosynthesis. The finally engineered strain BL21ΔfadD/pE-AtFatAssi2&pA-acc produced 82.6 mg/L free fatty acids (FFAs) under shake-flask conditions and FFAs yield on glucose reached about 3.3% of the theoretical yield. Two types of MUFAs, palmitoleate (16:1Δ9) and cis-vaccenate (18:1Δ11) made up more than 75% of the FFA profiles. Fed-batch fermentation of this strain further enhanced FFAs production to a titer of 1.27 g/L without affecting fatty acid compositions.
conclusionsThis study demonstrated the possibility to regulate fatty acid composition by using metabolic engineering approaches. FFAs produced by the recombinant E. coli strain consisted of high-level MUFAs and biodiesel manufactured from these fatty acids would be more suitable for current diesel engines.
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