ArticlePsychopharmacology2014
Effects of different ethanol-administration regimes on mRNA and protein levels of steroid 5α-reductase isozymes in prefrontal cortex of adolescent male rats.
Article in Psychopharmacology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 1 of them a synthesis that pooled it.
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Who cites it
7 citing papers in PubMed, 1 synthesis or guideline pooled it, 12 citations in OpenAlex.
- Adolescent Alcohol and Stress Exposure Rewires Key Cortical Neurocircuitry.Frontiers in neuroscience · 2022Pooled it
- Impact of impaired endogenous neurosteroidogenesis on outcomes following chronic alcohol exposure.bioRxiv : the preprint server for biology · 2026Article
- Voluntary elevated ethanol consumption in adolescent Sprague-Dawley rats: Procedural contributors and age-specificity.Alcohol (Fayetteville, N.Y.) · 2019Article
- Effects of adolescent alcohol consumption on the brain and behaviour.Nature reviews. Neuroscience · 2018Review
- Morphological and biochemical effects of weekend alcohol consumption in rats: Role of concentration and gender.World journal of hepatology · 2018Article
- Reversal learning and experimenter-administered chronic intermittent ethanol exposure in male rats.Psychopharmacology · 2016Article
- Adolescent alcohol exposure and persistence of adolescent-typical phenotypes into adulthood: a mini-review.Neuroscience and biobehavioral reviews · 2014Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
rationaleUnderage drinking is a leading public health problem in developed countries. An increasing proportion of adolescents consume alcoholic beverages every weekend. Increased anxiety, irritability, and depression among adolescents may induce them to seek for the anxiolytic and rewarding properties of alcohol. Allopregnanolone (AlloP) shares rewarding effects of ethanol and modulates ethanol intake. The rate-limiting enzyme in the biosynthesis of AlloP is steroid 5α-reductase (5α-R), which is expressed as three isozymes, 5α-R1, 5α-R2, and 5α-R3.
objectiveThe objective of this study was to quantify the expression levels of 5α-R isozymes in prefrontal cortex (PFC) of adolescent male rats after three different regimes of ethanol administration.
methodsAdolescent male Wistar rats were administered with ethanol (4 g/kg) or saline intraperitoneally for 1 day (acute), for 7 days (chronic), or every 72 h for 14 days (chronic intermittent). Messenger (m)RNA and protein levels of 5α-R isozymes were measured by quantitative RT-PCR and Western blot, respectively.
resultsEthanol significantly increased mRNA and protein levels of 5α-R1, 5α-R2, and 5α-R3 in the three different regimes of ethanol administration, being higher in the chronic intermittent regime in comparison with the others.
conclusionsThe expression of the AlloP-biosynthetic enzyme 5α-Rs increases in the prefrontal cortex of adolescent male rats under acute, chronic, and chronic intermittent regime of ethanol administration. The latter is very interesting because it mimics the teenage drinking behavior.
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