ArticlePloS one2014
Trypanosoma cruzi cell death induced by the Morita-Baylis-Hillman adduct 3-Hydroxy-2-methylene-3-(4-nitrophenylpropanenitrile).
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
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- Antifungal Effect and Inhibition of the Virulence Mechanism of D-Limonene againstMolecules (Basel, Switzerland) · 2022Article
- Leishmanicidal activity of Morita-Baylis-Hillman adducts.Parasitology research · 2022Article
- Selenium Derivatives as Promising Therapy for Chagas Disease:ACS infectious diseases · 2021Article
- Morita-Baylis-Hillman Adduct 2-(3-Hydroxy-2-oxoindolin-3-yl)acrylonitrile (ISACN) Modulates Inflammatory Process In vitro and In vivo.Inflammation · 2021Article
- Library of Selenocyanate and Diselenide Derivatives as In Vivo Antichagasic Compounds TargetingPharmaceuticals (Basel, Switzerland) · 2021Article
- Repositioning of leishmanicidal [1,2,3]Triazolo[1,5-a]pyridinium salts for Chagas disease treatment: Trypanosoma cruzi cell death involving mitochondrial membrane depolarisation and Fe-SOD inhibition.Parasitology research · 2020Article
- Article
- Biological activity of Morita-Baylis-Hillman adduct homodimers in L. infantum and L. amazonensis: anti-Leishmania activity and cytotoxicity.Parasitology research · 2019Article
- Insights into Chagas treatment based on the potential of bacteriocin AS-48.International journal for parasitology. Drugs and drug resistance · 2019Article
- New heteroleptic oxidovanadium(V) complexes: synthesis, characterization and biological evaluation as potential agents against Trypanosoma cruzi.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2018Article
- Ultrastructural and physiological changes induced by different stress conditions on the human parasite Trypanosoma cruzi.Cell stress & chaperones · 2017Article
- The in vitro biological activity of the Brazilian brown seaweed Dictyota mertensii against Leishmania amazonensis.Molecules (Basel, Switzerland) · 2014Article
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8 authors.
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Abstract
Chagas disease, caused by the protozoan Trypanosoma cruzi, remains a serious health concern due to the lack of effective vaccines or satisfactory treatment. In the search for new compounds against this neglected disease, we have previously demonstrated that the compound 3-Hydroxy-2-methylene-3-(4-nitrophenylpropanenitrile) (MBHA3), derived from the Morita-Baylis-Hillman reaction, effectively caused a loss of viability in both the epimastigote and trypomastigote forms. However, the mechanisms of parasite death elicited by MBHA3 remain unknown. The aim of this study was to better understand the morphophysiological changes and the mechanism of cell death induced by MBHA3 treatment on T. cruzi. To perform this analysis, we used confocal microscopy and flow cytometry to monitor the fluorescent probes such as annexin-V/propidium iodide (AV/PI), calcein-AM/ethidium homodimer (CA/EH), acridine orange (AO) and rhodamine 123 (Rho 123). Lower concentrations of MBHA3 led to alterations in the mitochondrial membrane potential and AO labeling, but did not decrease the viability of the epimastiogote forms, as determined by the CA/EH and AV/PI assays. Conversely, treatment with higher concentrations of MBHA3 led to extensive plasma membrane damage, loss of mitochondrion membrane potential, DNA fragmentation and acidification of the cytoplasm. Our findings suggest that at higher concentrations, MBHA3 induces T. cruzi epimastigote death by necrosis in a mitochondrion-dependent manner.
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