ArticleScientific reports2014
A color-tunable molecular beacon to sense miRNA-9 expression during neurogenesis.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Mir-30d Regulates Cardiac Remodeling by Intracellular and Paracrine Signaling.Circulation research · 2021Article
- DNA nanotechnology approaches for microRNA detection and diagnosis.Nucleic acids research · 2019Review
- Highly Sensitive in Vitro Diagnostic System of Pandemic Influenza A (H1N1) Virus Infection with Specific MicroRNA as a Biomarker.ACS omega · 2019Article
- Bioimaging of multiple piRNAs in a single breast cancer cell using molecular beacons.MedChemComm · 2017Article
- Article
- Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells.Scientific reports · 2017Article
- Monitoring the spatiotemporal activities of miRNAs in small animal models using molecular imaging modalities.International journal of molecular sciences · 2015Review
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A typical molecular beacon (MB) composing of a fluorophore and a quencher has been used to sense various intracellular biomolecules including microRNAs (miRNA, miR). However, the on/off-tunable miRNA MB is difficult to distinguish whether the observed low fluorescence brightness results from low miRNA expression or low transfection of the miRNA MB. We developed a color-tunable miRNA-9 MB (ColoR9 MB) to sense miR-9 expression-dependent color change. The ColoR9 MB was synthesized by a partially double-stranded DNA oligonucleotide containing a miR-9 binding site and a reporter probe with Cy3/black hole quencher 1 (BHQ1) at one end and a reference probe with Cy5.5 at the other end. The ColoR9 MB visualized CHO and P19 cells with red color in the absence of miR-9 and yellow color in the presence of miR-9. In vivo imaging demonstrated that the green fluorescence recovery of the reporter probe from the ColoR9 MB increased gradually during neuronal differentiation of P19 cells, whereas red fluorescence activity of the reference probe remained constant. These results showed the great specificity of sensing miR-9 expression- and neurogenesis-dependent color change.
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