Evidence map›Paper›PMID 24713513›Full record

ArticleToxicology and applied pharmacology2014

Environmentally persistent free radicals inhibit cytochrome P450 activity in rat liver microsomes.

James R Reed, George F Cawley, Taylor G Ardoin, Barry Dellinger, Slawomir M Lomnicki, Farhana Hasan, Lucy W Kiruri, Wayne L Backes

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Toxicology and applied pharmacology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 1 pooled it
1.3field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 1 synthesis or guideline pooled it, 33 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Characteristics and Risk Assessment of Environmentally Persistent Free Radicals (EPFRs) of PMInternational journal of environmental research and public health · 2023
    Article
  4. Article
  5. Article
  6. Review
  7. Article
  8. Article
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

James R ReedDepartment of Pharmacology and Experimental Therapeutics, Louisiana State University Health Science Center, 533 Bolivar St., New Orleans, LA 70112, USA; The Stanley S. Scott Cancer Center, Louisiana State University Health Science Center, 533 Bolivar St., New Orleans, LA 70112, USA. Electronic address: rreed@lsuhsc.edu.
George F CawleyDepartment of Pharmacology and Experimental Therapeutics, Louisiana State University Health Science Center, 533 Bolivar St., New Orleans, LA 70112, USA; The Stanley S. Scott Cancer Center, Louisiana State University Health Science Center, 533 Bolivar St., New Orleans, LA 70112, USA.
Taylor G ArdoinDepartment of Pharmacology and Experimental Therapeutics, Louisiana State University Health Science Center, 533 Bolivar St., New Orleans, LA 70112, USA; The Stanley S. Scott Cancer Center, Louisiana State University Health Science Center, 533 Bolivar St., New Orleans, LA 70112, USA.
Barry DellingerDepartment of Chemistry, Louisiana State University, Baton Rouge, LA 70803, USA.
Slawomir M LomnickiDepartment of Chemistry, Louisiana State University, Baton Rouge, LA 70803, USA.
Farhana HasanDepartment of Chemistry, Louisiana State University, Baton Rouge, LA 70803, USA.
Lucy W KiruriDepartment of Chemistry, Louisiana State University, Baton Rouge, LA 70803, USA.
Wayne L BackesDepartment of Pharmacology and Experimental Therapeutics, Louisiana State University Health Science Center, 533 Bolivar St., New Orleans, LA 70112, USA; The Stanley S. Scott Cancer Center, Louisiana State University Health Science Center, 533 Bolivar St., New Orleans, LA 70112, USA.
Louisiana State University · USLouisiana State University Health Sciences Center New Orleans · US

Funding

Role of microbiota in increased influenza severity due to particulate matter exposureP42ES013648 · NIEHS · LOUISIANA STATE UNIV A&M COL BATON ROUGE · PI SPRUNGER, PHILLIP · 2009 to 2024
$27.6M
TOXICOLOGICAL SIGNIFICANCE OF ALKYLBENZENE METABOLISMR01ES004344 · NIEHS · LSU HEALTH SCIENCES CENTER · PI BACKES, WAYNE L · 1996 to 2013
$4.0M
TOXICOLOGICAL SIGNIFICANCE OF ALKYLBENZENE METABOLISMR29ES004344 · NIEHS · LOUISIANA STATE UNIV HSC NEW ORLEANS · PI BACKES, WAYNE L · 1988 to 1992
–
NIEHS NIH HHS ES004344NIEHS NIH HHS ES013648NIEHS NIH HHS P42 ES013648NIEHS NIH HHS R01 ES004344
6 · The paper itself

Abstract

Combustion processes generate particulate matter that affects human health. When incineration fuels include components that are highly enriched in aromatic hydrocarbons (especially halogenated varieties) and redox-active metals, ultrafine particulate matter containing air-stable, environmentally persistent free radicals (EPFRs) is generated. The exposure to fine EPFRs (less than 2.5 μm in diameter) has been shown to negatively influence pulmonary and cardiovascular functions in living organisms. The goal of this study was to determine if these EPFRs have a direct effect on cytochrome P450 function. This was accomplished by direct addition of the EPFRs to rat liver microsomal preparations and measurement of several P450 activities using form-selective substrates. The EPFRs used in this study were formed by heating vapors from an organic compound (either monochlorophenol (MCP230) or 1,2-dichlorobenzene (DCB230)) and 5% copper oxide supported on silica (approximately 0.2 μm in diameter) to 230°C under vacuum. Both types of EPFRs (but not silica, physisorbed silica, or silica impregnated with copper oxide) dramatically inhibited the activities of CYP1A, CYP2B, CYP2E1, CYP2D2 and CYP3A when incubated at concentrations less than 0.1 mg/ml with microsomes and NADPH. Interestingly, at the same concentrations, the EPFRs did not inhibit HO-1 activity or the reduction of cytochrome c by NADPH-cytochrome P450 reductase. CYP2D2-selective metabolism by rat liver microsomes was examined in more detail. The inhibition of CYP2D2-selective metabolism by both DCB230- and MCP230-EPFRs appeared to be largely noncompetitive and was attenuated in the presence of catalase suggesting that reactive oxygen species may be involved in the mechanism of inhibition.

Indexed as

Cytochrome P-450 Enzyme InhibitorsAnimalsAryl Hydrocarbon HydroxylasesCatalaseChlorobenzenesChlorophenolsCytochrome P-450 Enzyme SystemDose-Response Relationship, DrugEnzyme InhibitorsFree RadicalsIsoenzymesKineticsMaleMicrosomes, LiverParticle SizeParticulate Matter2-dichlorobenzeneAryl Hydrocarbon HydroxylasesCatalaseChlorobenzenesChlorophenolsCyp2d2 protein, ratCytochrome P-450 Enzyme InhibitorsCytochrome P-450 Enzyme SystemEnzyme InhibitorsFree RadicalsIsoenzymesParticulate MatterCytochrome P450InhibitionMetabolismNanoparticleRadical

Identifiers

PMID24713513
PMCPMC4049543
OpenAlexW2024171130

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.