ArticleInvestigative ophthalmology & visual science2014
A novel pro-angiogenic function for interferon-γ-secreting natural killer cells.
Article in Investigative ophthalmology & visual science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 39 citations in OpenAlex.
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- Age-Related Macular Degeneration: A Disease of Cellular Senescence and Dysregulated Immune Homeostasis.Clinical interventions in aging · 2024Review
- The miR-183/96/182 cluster is a checkpoint for resident immune cells and shapes the cellular landscape of the cornea.The ocular surface · 2023Article
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- Innate immune biology in age-related macular degeneration.Frontiers in cell and developmental biology · 2023Review
- Immunomodulatory Cell Therapy Using αGalCer-Pulsed Dendritic Cells Ameliorates Heart Failure in a Murine Dilated Cardiomyopathy Model.Circulation. Heart failure · 2022Article
- Natural killer cell‑based immunotherapy for lung cancer: Challenges and perspectives (Review).Oncology reports · 2021Review
- Autologous cell therapy in diabetes‑associated critical limb ischemia: From basic studies to clinical outcomes (Review).International journal of molecular medicine · 2021Review
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- Resident Innate Immune Cells in the Cornea.Frontiers in immunology · 2021Review
- Lipid Keratopathy: A Review of Pathophysiology, Differential Diagnosis, and Management.Ophthalmology and therapy · 2020Review
- Cross talk between natural killer cells and mast cells in tumor angiogenesis.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2019Review
- Natural killer cells and other innate lymphoid cells in cancer.Nature reviews. Immunology · 2018Review
- B-cell leukemia/lymphoma 10 promotes angiogenesis in an experimental corneal neovascularization model.Eye (London, England) · 2018Article
- The Role of Limbal Epithelial Stem Cells in Regulating Corneal (Lymph)angiogenic Privilege and the Micromilieu of the Limbal Niche following UV Exposure.Stem cells international · 2018Review
- Animal models of ocular angiogenesis: from development to pathologies.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2017Review
- CXCL10 suppression of hem- and lymph-angiogenesis in inflamed corneas through MMP13.Angiogenesis · 2017Article
- Proangiogenic Function of T Cells in Corneal Transplantation.Transplantation · 2017Article
- T-helper-associated cytokines expression by peripheral blood mononuclear cells in patients with polypoidal choroidal vasculopathy and age-related macular degeneration.BMC ophthalmology · 2016Article
- Effect of Endometrial Injury on Secretion of Endometrial Cytokines and IVF Outcomes in Women with Unexplained Subfertility.Mediators of inflammation · 2015Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
Abstract
purposeTo explore the function of natural killer (NK) cells in inflammatory angiogenesis in mice.
methodsTo study ocular angiogenic responses we used the cornea BFGF micropellet and the laser-induced choroidal neovascularization (CNV) mouse models (C57BL/6). To deplete NK cells in these models, we injected an anti-NK1.1 antibody or an isotype antibody as a control. Corneas or choroids were immunohistochemically stained for blood vessels (CD31), macrophages (F4/80), or CNV (isolectin-IB4). Vascular endothelial growth factors (VEGF), IFN-γ, or TNF-α levels were measured by real-time quantitative PCR (qPCR) or flow cytometry. A coculture assay of macrophages, NK cells, and human umbilical vein endothelial cells (HUVECs) was analyzed morphometrically to examine the ability of NK cells to induce angiogenesis in vitro.
resultsOur data demonstrate that in vivo depletion of NK cells leads to a significant reduction of corneal angiogenesis and CNV. Furthermore, NK cell depletion reduces macrophage infiltration into the cornea and mRNA expression levels of VEGF-A, VEGF-C, and VEGFR3 at day 7 after micropellet insertion. In the laser-induced CNV model, our data show that NK cell depletion leads to decreased areas of CNV and significantly reduced mRNA expression of VEGFs and IFN-γ in the choroid. An in vitro coculture assay shows an IFN-γ-dependent increase in VEGF expression levels, thereby increasing endothelial cell proliferation.
conclusionsOur findings demonstrate a novel pro-angiogenic function for NK cells, indicating that IFN-γ-secreting NK cells can induce angiogenesis by promoting enhanced VEGF expression by macrophages.
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Registered trials
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