ArticleCNS neuroscience & therapeutics2014
In vivo two-photon fluorescence microscopy reveals disturbed cerebral capillary blood flow and increased susceptibility to ischemic insults in diabetic mice.
Article in CNS neuroscience & therapeutics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- Assessing cerebral capillary function and stalling using single capillary reporters in ultrasound localization microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Cortical microvascular blood flow velocity mapping by combining dynamic light scattering optical coherence tomography and two-photon microscopy.Journal of biomedical optics · 2023Article
- Three-dimensional visualization of cerebral blood vessels and neural changes in thick ischemic rat brain slices using tissue clearing.Scientific reports · 2022Article
- Diamond Raman laser and Yb fiber amplifier forBiomedical optics express · 2022Article
- High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics.Nature communications · 2020Article
- Role of pericyte-derived SENP1 in neuronal injury after brain ischemia.CNS neuroscience & therapeutics · 2020Article
- Improved collateral flow and reduced damage after remote ischemic perconditioning during distal middle cerebral artery occlusion in aged rats.Scientific reports · 2020Article
- Impaired Collateral Flow in Pial Arterioles of Aged Rats During Ischemic Stroke.Translational stroke research · 2020Article
- Article
- Cerebral microvascular dysfunction and neurodegeneration in dementia.Stroke and vascular neurology · 2019Review
- Cathepsin B inhibition ameliorates leukocyte-endothelial adhesion in the BTBR mouse model of autism.CNS neuroscience & therapeutics · 2019Article
- Transit time homogenization in ischemic stroke - A novel biomarker of penumbral microvascular failure?Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2018Article
- Impact of Metabolic Diseases on Cerebral Circulation: Structural and Functional Consequences.Comprehensive Physiology · 2018Review
- Histidine Alleviates Impairments Induced by Chronic Cerebral Hypoperfusion in Mice.Frontiers in physiology · 2018Article
- Multiparametric Imaging of Organ System Interfaces.Circulation. Cardiovascular imaging · 2017Review
- Characterization of Emodin as a Therapeutic Agent for Diabetic Cataract.Journal of natural products · 2016Article
- Increasing the Permeability of the Blood-brain Barrier in Three Different Models in vivo.CNS neuroscience & therapeutics · 2015Article
- Aldose reductase inhibition alleviates hyperglycemic effects on human retinal pigment epithelial cells.Chemico-biological interactions · 2015Article
- P2RX7 sensitizes Mac-1/ICAM-1-dependent leukocyte-endothelial adhesion and promotes neurovascular injury during septic encephalopathy.Cell research · 2015Article
- Hyperglycaemia does not increase perfusion deficits after focal cerebral ischaemia in male Wistar rats.Brain and neuroscience advancesArticle
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Authors and funding
13 authors at 4 institutions in 3 countries.
Funding
Abstract
aimsDiabetes mellitus increases the risk of stroke, but the mechanisms are unclear. The present study tested the hypothesis that diabetes mellitus disturbs the brain microcirculation and increases the susceptibility to cerebral damage in a middle cerebral artery occlusion (MCAO) model of ischemia.
methodsDiabetes was induced by streptozocin in mice expressing green fluorescent protein in endothelial cells (Tie2-GFP mice). Four weeks later, they were subjected to transient (20 min) MCAO. In vivo blood flow was measured by two-photon laser-scanning microscopy (TPLSM) in cerebral arteries, veins, and capillaries.
resultsThere was a significant decrease in red blood cell (RBC) velocity in capillaries in diabetic mice as assessed by TPLSM, yet the regional cerebral blood flow, as assessed by laser Doppler flowmetry, was maintained. Brain capillary flow developed turbulence after MCAO only in diabetic mice. These mice sustained increased neurological deficits after MCAO which were accompanied by an exaggerated degradation of tight junction proteins and blunted CaMKII phosphorylation in cerebral tissues indicating disruption of the blood-brain barrier and disturbed cognitive potential.
conclusionDiabetic mice are more susceptible to disturbances of cerebral capillary blood flow which may predispose them to neurovascular defects following ischemia.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.